FEI Tecnai T12 Biotwin

DTU Nanolabs Tecnai T12 is a transmission electron microscope (TEM) used for performing both conventional imaging and diffraction, energy dispersive X-ray spectrosocopy (EDS) and cryo experiments. It is a versatile instrument, ideal for studying a wide range of general and advanced materials, soft matter, composites, etc.

The Tecnai T12 is a transmission electron microscope equipped with a thermionic electron source with a Lab6 filament and acceleration voltages that can vary between 80-120 kV. It has the FEI bio-twin pole piece with a pole piece gap of 11 mm (Cs about 2.2 mm). This gives the microscope a resolution @ 120 kV of above 4 Å in TEM mode. Additionally, it is equipped with permanent cryo plates, which makes it ideal for cryo experiments.

On the analytical side, the microscope is equipped with an Oxford X-Max 80T SDD X-ray detector for Energy Dispersive X-ray (EDS) spectroscopy. For imaging, the microscope is equipped with Gatan Orius SC1000 CCD (4008x2672 px).

Sample holders

The default specimen holder for the T12 is called Single-Tilt (FEI ST) and it is always present in the microscope room.
The lab has also other specimen holders used for various application e.g. heating (furnace and MEMS-based), cooling, biasing and tomography. For information on the various specimen holders see HERE

Who may operate the Tecnai T12

Anybody in need of a TEM microscope is welcome to apply for access to our facility by submitting an Access Request Form .Shortly after your ARF has been received you will be contact by the microscopy support team in order to set up a meeting to further discuss your application.
In order to be authorised to use the Tecnai T12 you must complete the Tecnai training with consists of a theoretical lecture and 3 practical sessions of 4 hours each.
Due to the similarity of the basic operations on a Tecnai TEM and a Titan TEM, a Tecnai trained user can receive additional training and progress in using a Titan TEM.

When you have demonstrated a high level of familiarity with the Tecnai TEM you will be authorized and allowed to book it via our booking system called LabManager.

Booking

Booking on the T12 is done by the users in accordance to the booking rules. At the moment, there are NO booking rules on the T12.

Further information

TEM Tecnai T12 in LabManager

Calibration

Magnification calibration cameras

Process information

The following techniques and processes are available on the microscope (list isn't complete):

Techniques:

EDS/WDS

Processes:

Cryo-TEM

Tips and Tricks

Here you can find some help to solve small issues on the microscope. Let us know, if you have other suggestions.

Reference material

Books:

L. Reimer, Transmission Electron Microscopy - Physics of image formation and microanalysis (Springer, 1997).

David B. Williams, C. Barry Carter, Transmission Electron Microscopy - A Textbook for Materials Science (Springer, 2009).

Online Material:

Tutorials in Transmission Electron Microscopy by John Rodenburg

TEM Basics by University of Liverpool

TEM Introduction by ammrf