LabAdviser/314/Preparation 314-307/Soft-matter: Difference between revisions
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The main steps of this technique is as follows:<br /> | The main steps of this technique is as follows:<br /> | ||
1. The sample is spread | 1. The sample is spread onto a glow discharged EM grid <br /> | ||
2. The liquid droplet is then blotted with filter paper until only a very thin film of fluid remains <br /> | 2. The liquid droplet is then blotted with filter paper until only a very thin film of fluid remains <br /> | ||
3. The grid is then rapidly plunged into a cryogen (usually liquid ethane) <br /> | 3. The grid is then rapidly plunged into a cryogen (usually liquid ethane) <br /> | ||