LabAdviser/314/Microscopy 314-307/TEM/ETEM/OneView: Difference between revisions
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=== | === Image acquisition === | ||
1. Choose '''TEM imaging''' under '''Technique Manager''' for general image acquisition.<br /> | 1. Choose '''TEM imaging''' under '''Technique Manager''' for general image acquisition.<br /> | ||
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* '''Correct Drift''' aligns this stack of individual frames.<br /> | * '''Correct Drift''' aligns this stack of individual frames.<br /> | ||
* This means the signal-to-noise in each individual frame should be high enough to get a decent summed final image.<br /> | * This means the signal-to-noise in each individual frame should be high enough to get a decent summed final image.<br /> | ||
==== Diffraction mode ==== | ==== Diffraction mode ==== | ||
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* Increasing the exposure results in summing individual frames (as in imaging mode).<br /> | * Increasing the exposure results in summing individual frames (as in imaging mode).<br /> | ||
* As the minimum exposure time for the individual frame is 4x higher, the signal-to-noise in the individual frame is better compared to imaging mode. This can help for very low dose acquisition.<br /> | * As the minimum exposure time for the individual frame is 4x higher, the signal-to-noise in the individual frame is better compared to imaging mode. This can help for very low dose acquisition.<br /> | ||
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=== ''in situ'' acquisition === | === ''in situ'' acquisition === | ||