LabAdviser/314/Preparation 314-307: Difference between revisions
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==Plunger== | ==Plunger== | ||
The FEI Vitrobot™ is used for vitrifying (freezing) single particle samples for cryo-TEM. The Vitrobot™ is fully automated and performs the cryo-fixation process at constant conditions (temperature, relative humidity, blotting and freezing velocity), which ensures high quality cryo-fixation. The sample is pipetted on a TEM grid, blotted and then plunged in liquid ethane, which is cooled with liquid nitrogen. The FEI Vitrobot™ Data Sheet is here [[:File:Vitrobot_Mark_IV_Datasheet.pdf]] and an example of the APV can be found here [[:File:APV_Vitrobot.pdf]]. | <!--The FEI Vitrobot™ is used for vitrifying (freezing) single particle samples for cryo-TEM. The Vitrobot™ is fully automated and performs the cryo-fixation process at constant conditions (temperature, relative humidity, blotting and freezing velocity), which ensures high quality cryo-fixation. The sample is pipetted on a TEM grid, blotted and then plunged in liquid ethane, which is cooled with liquid nitrogen. The FEI Vitrobot™ Data Sheet is here [[:File:Vitrobot_Mark_IV_Datasheet.pdf]] and an example of the APV can be found here [[:File:APV_Vitrobot.pdf]]. | ||
The Vitrobot™ is located in building 314, in the Soft Mater (toxic) Lab. For further information on the machine usage contact ramona.mateiu@cen.dtu.dk. | The Vitrobot™ is located in building 314, in the Soft Mater (toxic) Lab. For further information on the machine usage contact ramona.mateiu@cen.dtu.dk. | ||
[[File:Vitrobot.jpg|200px|thumb|left|DTU Cens FEI Vitrobot]]<br /> | [[File:Vitrobot.jpg|200px|thumb|left|DTU Cens FEI Vitrobot]]<br /> --> | ||
The Leica plunge freezer is used to vitrifying (freezing) samples for cryo-TEM under liquid ethane temperature. | |||
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