Jump to content

LabAdviser/314/Preparation 314-307/Soft-matter: Difference between revisions

Mktracy (talk | contribs)
Mktracy (talk | contribs)
No edit summary
Line 30: Line 30:
<br clear="all" />
<br clear="all" />


<!--= High Pressure Freezer =
= High Pressure Freezer =
== Leica EM ICE ('''COMING SOON''') ==
== Leica EM ICE ==
High pressure freezing is key for the study of intricate changes in fine structure or cellular dynamics.
High pressure freezing is key for the study of intricate changes in fine structure or cellular dynamics.
* Cryo-immobilize your aqueous samples under high pressure with a unique freezing principle and uncover secrets of the cellular process.
* Cryo-immobilize your aqueous samples under high pressure with a unique freezing principle and uncover secrets of the cellular process.
Line 53: Line 53:


= Freeze substitution =
= Freeze substitution =
== Leica EM AFS2 and EM FSP ('''COMING SOON''') ==
== Leica EM AFS2 and EM FSP ==
The Leica EM AFS2 performs freeze substitution and progressive lowering of temperature (PLT) techniques and allows low temperature embedding and polymerization of resins. The Leica EM FSP (freeze substitution processor), an automatic reagent handling system combined with the Leica EM AFS2, dispenses reagents for both freeze substitution and PLT applications. The LED illumination from within the chamber and the attached stereomicroscope for viewing and positioning of samples ensures ease of use.
The Leica EM AFS2 performs freeze substitution and progressive lowering of temperature (PLT) techniques and allows low temperature embedding and polymerization of resins. The Leica EM FSP (freeze substitution processor), an automatic reagent handling system combined with the Leica EM AFS2, dispenses reagents for both freeze substitution and PLT applications. The LED illumination from within the chamber and the attached stereomicroscope for viewing and positioning of samples ensures ease of use.


Line 64: Line 64:


= Microtome =
= Microtome =
== RMC MT-7 Microtome ==
Our RMC MT-7 Microtome is a vintage piece of equipment and can be used for specimen sample preparation for SEM/EDX analysis and/or for cutting relative thin slices (approx. 200nm thick) for TEM analysis. First, the specimen needs to be embedded into a resin or an epoxy, and then can be sectioned using a glass or diamond knife. 
[[File:RMC microtome.jpg|400px|thumb|left|RMC MT-7 Microtome Location at DTU Nanolab Building 307 Room 906]]<br clear="all" />
For further information about the microtome usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
<br clear="all" />
== Leica EM UC7 Ultramicrotome==
== Leica EM UC7 Ultramicrotome==
The Ultramicrotome Leica EM UC7 provides easy sample preparation of ultrathin sections for TEM, SEM, AFM and LM application. It is suitable for highly skilled or absolute beginners. The Leica ultramicrotome can provide ultra thin sectioning of sample embedded in a resin/epoxy block with a feed range of 1 nm up to 15 µm. Glass or diamond knife can be used for the ultra sectioning of the samples.  
The Ultramicrotome Leica EM UC7 provides easy sample preparation of ultrathin sections for TEM, SEM, AFM and LM application. It is suitable for highly skilled or absolute beginners. The Leica ultramicrotome can provide ultra thin sectioning of sample embedded in a resin/epoxy block with a feed range of 1 nm up to 15 µm. Glass or diamond knife can be used for the ultra sectioning of the samples.