LabAdviser/314/Microscopy 314-307/SEM/QFEG: Difference between revisions
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== STEM (Scanning Transmission Electron Microscopy) == | == STEM (Scanning Transmission Electron Microscopy) == | ||
STEM combines the principles of transmission electron microscopy and scanning electron microscopy and can be performed on either type of instrument. In the STEM setting, the sample requires to have a thickness of less than 200 nm for visualization. | STEM combines the principles of transmission electron microscopy and scanning electron microscopy and can be performed on either type of instrument. In the STEM setting, the sample requires to have a thickness of less than 200 nm for visualization. Sample preparation prior to microscopy involve samples being embedded in epoxy resin and then ultra section using the ultramicrotome. Thin sections at cryogenic temperatures (below −180 °C) can also be done at Nanolab. | ||
[[File:20210519_Microalgae_K2_007-1.jpg|500px|left|thumb|STEM imaging of a cross section of Microalgae, 100 nm thick section]]<br clear="all" /> | [[File:20210519_Microalgae_K2_007-1.jpg|500px|left|thumb|STEM imaging of a cross section of Microalgae, 100 nm thick section]]<br clear="all" /> | ||