LabAdviser/314/Microscopy 314-307/TEM/ETEM/OneView: Difference between revisions
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2. Options:<br /> | 2. Options:<br /> | ||
* '''I''' for imaging mode, '''D''' for diffraction mode; binning from | * '''I''' for imaging mode, '''D''' for diffraction mode; binning from 4096px down to 512px (when changing mode/binning, wait a minute for the electronics to stabilize again)<br /> | ||
* '''Live''' and '''Capture''' to follow the sample on the camera and acquire individual images.<br /> | * '''Live''' and '''Capture''' to follow the sample on the camera and acquire individual images.<br /> | ||
* The cogwheel icon opens additional options like auto save and how drift corrected images are handled.<br /> | * The cogwheel icon opens additional options like auto save and how drift corrected images are handled.<br /> | ||
* '''Correct Drift''' is the drift correction between individual frame acquisition. It adds a cyan window to the Live View window, which can be moved. Drift correction is based on this section of the image.<br /> | |||
==== Imaging mode ==== | |||
* The camera is running on the maximum frame rate (minimum exposure time) for the selected binning. For 4k it means, each individual frame is acquired at about 0.04s (25fps).<br /> | |||
* Changing the '''Exposure''' (no matter if in View of Capture mode) means, the camera acquires individual frames with the shortest possible exposure time, and stacks them up until the selected exposure time is reached. So, it's not the "real" exposure time on the camera which is changed, but the number of individual frames in the stacked image.<br /> | |||
* '''Correct Drift''' aligns these stacked individual frames.<br /> | |||
* This means the signal-to-noise in each individual frame should be high enough to get a decent stacked final image.<br /> | |||
==== Diffraction mode ==== | |||
* | |||
=== ''in situ'' acquisition === | === ''in situ'' acquisition === | ||