LabAdviser/314/Preparation 314-307/Soft-matter: Difference between revisions
| Line 84: | Line 84: | ||
== Leica EM FC7 Cryo-Ultramicrotome == | == Leica EM FC7 Cryo-Ultramicrotome == | ||
The main steps of this technique proceed as follows:<br /> | |||
1. Set up the cryo chamber EM FC7 to the EM UC7 apparatus <br /> | |||
2. Mount both the trim and sectioning diamond knives to the knive holder and set up in the cryo chamber <br /> | |||
3. Fill up liquid nitrogen Dewar <br /> | |||
4. Set pump between the liquid nitrogen Dewar and the ultramicrotome apparatus <br /> | |||
5. Let equipment to cool down for 1 hour <br /> | |||
6. Meanwhile prepare samples <br /> | |||
7. Mount sample <br /> | |||
8. Trimming and ultrasectioning of sample <br /> | |||
9. Collecting sections and transfer to an EM grid <br /> | |||
Details on the procedure is illustrated as follows: | Details on the procedure is illustrated as follows: | ||
*[[/Cryo-Ultramicrotomy|Procedure steps]]<br /> | *[[/Cryo-Ultramicrotomy|Procedure steps]]<br /> | ||
Operating manual: [[:File:EM FC7 Operating manual.pdf]]<br /> | |||
Risk Assessment: [[:File:Risk assessment - EM FC7 Cryo-Ultramicrotome.pdf]]<br /> | |||