LabAdviser/314/Microscopy 314-307/TEM/T20

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Tecnai T20 in building 314.

FEI Tecnai T20

DTU Nanolabs Tecnai T20 is a transmission electron microscope (TEM) used for performing both conventional, high resolution and scanning transmission microscopy (STEM) measurements. It is a versatile instrument, ideal for studying a wide range of general and advanced materials, soft matter, composites, etc. The microscope is equipped to carry out chemical analysis as well as Cryo-TEM for soft materials.

The Tecnai T20 is a transmission electron microscope equipped with a thermionic electron source with a Lab6 filament and acceleration voltages that can vary between 80-200 kV. This gives the microscope a resolution @ 200 kV of 1.4 Å in TEM mode. Other tecniques available on the microscope are scanning transmission electron microscopy (STEM, resolution 10 Å) with a high-angle annular dark-field detector (HAADF).

On the analytical side, the microscope is equipped with an Oxford X-Max 80T SDD X-ray detector for Energy Dispersive X-ray (EDS) spectroscopy and a Gatan Tridiem 863 imaging filter allowing for electron energy-loss spectroscopy (EELS) and energy-filtered imaging. For imaging, the microscope is equipped with TVIPS XF416 CCD before the the energy filter and a GATAN Ultrascan US1000 after the energy filter.

TEM Schematic

A general schematic of a transmission electron microscope can be found here

Sample holders

The default specimen holder for the T20 is called Single-Tilt (FEI ST) and it is always present in building 314 Prep Room1.
The lab has also other specimen holders used for various application e.g. heating (furnace and MEMS-based), cooling, biasing and tomography. For information on the various specimen holders see HERE

Who may operate the Tecnai T20

Anybody in need of a TEM microscope is welcome to apply for access to our facility by submitting an Access Request Form .Shortly after your ARF has been received you will be contact by the microscopy support team in order to set up a meeting to further discuss your application.
In order to be authorised to use the Tecnai T20 you must compete the Tecnai training with consists of a theoretical lecture and 3 practical sessions of 4 hours each.
Due to the similarity of the basic operations on a Tecnai TEM and a Titan TEM, a Tecnai trained user can receive additional training and progress in using a Titan TEM.

When you have demonstrated a high level of familiarity with the Tecnai TEM you will be authorized and allowed to book it via our booking system called LabManager.

Booking

Booking on the T20 is done by the users in accordance to the booking rules. Booking rules can be found in LabManager under "Documents" for the T20.

Further information

TEM Tecnai T20 in LabManager

Calibration

• Magnification calibration cameras
• Collection angles GIF
• Convergence angles STEM

Process information

The following techniques and processes are available on the microscope (list isn't complete):

Techniques:

• Bright Field Imaging
• Scanning Transmission Electron Microscopy
• Diffraction
• EDS/WDS
• EELS
• EFTEM

Tips and Tricks

Here you can find some help to solve small issues on the microscope. Let us know, if you have other suggestions.

• GIF apertures are not responding

Reference material

Books:

L. Reimer, Transmission Electron Microscopy - Physics of image formation and microanalysis (Springer, 1997).

David B. Williams, C. Barry Carter, Transmission Electron Microscopy - A Textbook for Materials Science (Springer, 2009).

Online Material:

Tutorials in Transmission Electron Microscopy by John Rodenburg

TEM Basics by University of Liverpool

TEM Introduction by ammrf