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== Leica EM GP2 ==
== Leica EM GP2 ==
Plunge freezing is a cryo-fixation method used to preserve samples in their native state prior to cryo-electron microscopy. The EM GP2 plunge freezes fluid or extremely thin samples spread on an electron microscopy grid into liquid ethane and afterwards excess fluid is removed by automatic blotting. Prior to freezing, the sample is maintained in a temperature and humidity controlled environmental chamber which is adjustable between +4°C and +60°C and room humidity to 99 %.  
Plunge freezing is a cryo-fixation method used to preserve samples in their native state prior to cryo-electron microscopy. The EM GP2 plunge freezes fluid or extremely thin samples spread on an electron microscopy grid into liquid ethane and afterwards excess fluid is removed by automatic blotting. Prior to freezing, the sample is maintained in a temperature and humidity controlled environmental chamber which is adjustable between +4°C and +60°C and room humidity to 99 %.  
The main steps of this technique is as follows:<br />
1. The sample is spread onto a glow discharged EM grid <br />
2. The liquid droplet is then blotted with filter paper until only a very thin film of fluid remains <br />
3. The grid is then rapidly plunged into a cryogen (usually liquid ethane) <br />
4. The grid is afterwards stored in a grid box submerged in liquid nitrogen until finally loaded into the cryo-electron microscope for imaging <br />




[https://labmanager.dtu.dk/view_binary.php?class=MiscDocument&id=15&name=Leica_EM_GP2_Operating_manual.pdf Operating manual] ''- requires login''<br />  
[https://labmanager.dtu.dk/view_binary.php?class=MiscDocument&id=15&name=Leica_EM_GP2_Operating_manual.pdf Operating manual] ''- requires login''<br />  


[[File:Plunge Freezer (2).jpg|400px|left|thumb|Leica EM GP2 Location at DTU Nanolab Building 314 Room 014 (Tecnai T20 room)]]<br clear="all" />
[[File:IMG_9460_GP2.jpg|400px|left|thumb|Leica EM GP2 Location at DTU Nanolab Building 307 Room 909B]]<br clear="all" />


For further information on the equipment usage or training contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].  
For further information on the equipment usage or training contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].  
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<!--= High Pressure Freezer =
= High Pressure Freezer =
== Leica EM ICE ('''COMING SOON''') ==
== Leica EM ICE ==
High pressure freezing is key for the study of intricate changes in fine structure or cellular dynamics.
High pressure freezing is key for the study of intricate changes in fine structure or cellular dynamics.
* Cryo-immobilize your aqueous samples under high pressure with a unique freezing principle and uncover secrets of the cellular process.
* Cryo-immobilize your aqueous samples under high pressure with a unique freezing principle and uncover secrets of the cellular process.
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[[File:HPF EM ICE.jpg|600px|left|thumb|Leica EM ICE Location at DTU Nanolab Building 307]]<br clear="all" />
[[File:HPF EM ICE.jpg|600px|left|thumb|Leica EM ICE Location at DTU Nanolab Building 307 Room 909B]]<br clear="all" />
For further information on the equipment usage or training contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
For further information on the equipment usage or training contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].


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= Freeze substitution =
= Freeze substitution =
== Leica EM AFS2 and EM FSP ('''COMING SOON''') ==
== Leica EM AFS2 and EM FSP ==
The Leica EM AFS2 performs freeze substitution and progressive lowering of temperature (PLT) techniques and allows low temperature embedding and polymerization of resins. The Leica EM FSP (freeze substitution processor), an automatic reagent handling system combined with the Leica EM AFS2, dispenses reagents for both freeze substitution and PLT applications. The LED illumination from within the chamber and the attached stereomicroscope for viewing and positioning of samples ensures ease of use.
The Leica EM AFS2 performs freeze substitution and progressive lowering of temperature (PLT) techniques and allows low temperature embedding and polymerization of resins. The Leica EM FSP (freeze substitution processor), an automatic reagent handling system combined with the Leica EM AFS2, dispenses reagents for both freeze substitution and PLT applications. The LED illumination from within the chamber and the attached stereomicroscope for viewing and positioning of samples ensures ease of use.




[[File:Freeze-substitution EM AFS2.jpg|300px|left|thumb|Leica EM AFS2 Location at DTU Nanolab Building 307]]
[[File:Freeze-substitution EM AFS2.jpg|300px|left|thumb|Leica EM AFS2 Location at DTU Nanolab Building 307 Room 909B]]
[[File:EM FSP.JPG|200px|left|thumb|Leica EM FSP Location at DTU Nanolab Building 307]]<br clear="all" />
[[File:EM FSP.JPG|200px|left|thumb|Leica EM FSP Location at DTU Nanolab Building 307 Room 909B]]<br clear="all" />
For further information on the equipment usage or training contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
For further information on the equipment usage or training contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].


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= Microtome =
= Microtome =
== RMC MT-7 Microtome ==
Our RMC MT-7 Microtome is a vintage piece of equipment and can be used for specimen sample preparation for SEM/EDX analysis and/or for cutting relative thin slices (approx. 200nm thick) for TEM analysis. First, the specimen needs to be embedded into a resin or an epoxy, and then can be sectioned using a glass or diamond knife. 
[[File:RMC microtome.jpg|400px|thumb|left|RMC MT-7 Microtome Location at DTU Nanolab Building 307 Room 906]]<br clear="all" />
For further information about the microtome usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
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== Leica EM UC7 Ultramicrotome==
== Leica EM UC7 Ultramicrotome==
The Ultramicrotome Leica EM UC7 provides easy sample preparation of ultrathin sections for TEM, SEM, AFM and LM application. It is suitable for highly skilled or absolute beginners. The Leica ultramicrotome can provide ultra thin sectioning of sample embedded in a resin/epoxy block with a feed range of 1 nm up to 15 µm. Glass or diamond knife can be used for the ultra sectioning of the samples.  
The Ultramicrotome Leica EM UC7 provides easy sample preparation of ultrathin sections for TEM, SEM, AFM and LM application. It is suitable for highly skilled or absolute beginners. The Leica ultramicrotome can provide ultra thin sectioning of sample embedded in a resin/epoxy block with a feed range of 1 nm up to 15 µm. Glass or diamond knife can be used for the ultra sectioning of the samples.  




The main steps of this technique proceed as follows:<br />
[https://labmanager.dtu.dk/view_binary.php?class=MiscDocument&id=15&name=EM_UC7_Operating_manual.pdf Operating manual] ''-requires login''<br />
1. Mount sample <br />
2. Mount glass or diamond knife to knife holder <br />
3. Trimming or ultrasectioning of sample <br />
4. Fishing sections <br />
5. Transfer to an EM grid <br />


[[File:IMG_9461_UC7.jpg|400px|left|thumb|Leica EM UC7 Location at DTU Nanolab Building 307 Room 906]]<br clear="all" />


[https://labmanager.dtu.dk/view_binary.php?class=MiscDocument&id=15&name=EM_UC7_Operating_manual.pdf Operating manual] ''-requires login''<br />
'''Requirement for training:'''  
* Completion of the DTU Epoxy course
* Purchased of own diamond knife.


[[File:Leica EM UC7.jpg|400px|left|thumb|Leica EM UC7 Location at DTU Nanolab Building 307 Room 906]]<br clear="all" />


'''Requirement for training:''' Completion of the DTU Epoxy course and purchased of own diamond knife. For further information about the equipment usage or training contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
For further information about the equipment usage or training contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].


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The main steps of this technique proceed as follows:<br />
[https://labmanager.dtu.dk/view_binary.php?class=MiscDocument&id=15&name=EM_FC7_Operating_manual.pdf Operating manual] ''- requires login''<br />  
1. Set up the cryo chamber EM FC7 to the EM UC7 apparatus <br />
2. Mount both the trim and sectioning diamond knives to the knive holder and set up in the cryo chamber <br />
3. Connect the pump tube between the liquid nitrogen Dewar and the ultramicrotome apparatus <br />
4. Fill up liquid nitrogen Dewar <br />
5. Let equipment to cool down for 1 hour <br />
6. Meanwhile prepare samples (Cut big samples to smaller pieces) <br />
7. Freeze sample in liquid nitrogen and mount sample <br />
8. Trimming and ultrasectioning of sample <br />
9. Collecting sections and transfer to an EM grid <br />


[[File:Leica EM FC7 Cryo-Ultramicrotome.jpg|400px|left|thumb|Leica EM FC7 Location at DTU Nanolab Building 307 Room 906]]<br clear="all" />


[https://labmanager.dtu.dk/view_binary.php?class=MiscDocument&id=15&name=EM_FC7_Operating_manual.pdf Operating manual] ''- requires login''<br />
'''Requirement for training:'''  
* Purchased of own diamond knife.


[[File:Leica EM FC7 Cryo-Ultramicrotome.jpg|400px|left|thumb|Leica EM FC7 Location at DTU Nanolab Building 307 Room 906]]<br clear="all" />


'''Requirement for training:''' Purchased of own diamond knife. For further information about the equipment usage or training contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
For further information about the equipment usage or training contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].


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= KnifeMaker=
= KnifeMaker=
== LKB Knifemaker 7801A ==
== Leica EM KMR3 ==
At DTU Nanolab we offer the possibility to make glass knives for ultramicrotomy applications using the LKB Knifemaker 7801A.
At DTU Nanolab we offer the possibility to make glass knives for ultramicrotomy applications using the Leica EM KMR3 Knifemaker.




[https://labmanager.dtu.dk/view_binary.php?class=MiscDocument&id=15&name=LKB_Knifemaker_7800B_Manual.pdf Manual of LKB Knifemaker 7801A] ''- requires login'' <br />


[[File:Knifemaker.jpg|300px|thumb|left|LKB Knifemaker Location at DTU Nanolab Building 307 Room 906]]<br clear="all" />
[[File:IMG_9462_KMR3.jpg|400px|thumb|left|LKB Knifemaker Location at DTU Nanolab Building 307 Room 909B]]<br clear="all" />
For further information about the knifemaker usage and training contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
For further information about the knifemaker usage and training contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].


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[https://labmanager.dtu.dk/view_binary.php?class=MiscDocument&id=15&name=EM_CPD300_Operating_manual_small.pdf Operating manual] ''- requires login'' <br />  
[https://labmanager.dtu.dk/view_binary.php?class=MiscDocument&id=15&name=EM_CPD300_Operating_manual_small.pdf Operating manual] ''- requires login'' <br />  


[[File:EM CPD300.jpg|300px|thumb|left|Leica EM CPD300 Location at DTU Nanolab Building 314 Room 040]]<br clear="all" />
[[File:IMG_9456_CPD300.jpg|400px|thumb|left|Leica EM CPD300 Location at DTU Nanolab Building 307 Room 903]]<br clear="all" />
For further information on the CPD usage or training contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
For further information on the CPD usage or training contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].


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= Drying oven =
= Coater =
The TS9026 drying oven is of 26L capacity and is used for epoxy embedding experiments.
== Leica EM ACE600 ==
 
The coater offers 3 applications
* Platinum coating
* Glow discharge
* Carbon coating


Specifications:
* Temperature range is from 2°C to 250°C
* Fan speed is 0 to 10
* Capacity is 26L


[[File:IMG 9634.jpg|400px|thumb|left|TS9026 Drying oven Location at DTU Nanolab Building 307 Room 903]]<br clear="all" />
[[File:IMG_9459_ACE600.jpg|400px|thumb|left|Leica EM ACE600 Location at DTU Nanolab Building 307 Room 907]]<br clear="all" />
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
For further information about the coater usage and training contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
 
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= Sonic Dismembrator =
The Fisherbrand Model 505 sonic dismembrator apparatus applies sound energy to agitate particles in a sample. For biological applications, sonication may be sufficient to disrupt or deactivate a biological material. For example, sonication is often used to disrupt cell membranes and release cellular contents. In nanotechnology sonication is commonly used for evenly dispersing nanoparticles in liquids.
 
[[File:IMG_9633.jpg|400px|thumb|left|Fisherbrand Model 505 Sonic Dismembrator Location at DTU Nanolab Building 314 Room 040]]<br clear="all" />
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].


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[[File:IMG_9636.jpg|400px|thumb|left|Mini tube rotator Location at DTU Nanolab Building 307 Room 903]]<br clear="all" />
[[File:IMG_9636.jpg|400px|thumb|left|Mini tube rotator Location at DTU Nanolab Building 307 Room 903]]<br clear="all" />
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
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== Centrifuge 5430R ==
Two rotor are available for this centrifuge in the Softmatter lab.
* Rotor FA-45-30-11
Allows max. capacity 30 micro test tubes for 1.5/
2.0 mL and operates at 25000 ×g /15350 rpm
* Rotor F-35-6-30
Allows max. capacity 6 conical tubes for 50 mL or 6 conical tubes for 15 mL and operates at 7745 ×g /7830 rpm
'''Specifications:'''
'''Specifications:'''
* Max. rotor capacity depending on type of rotor: 30 × 1.5/2.0 mL, 6 × 50 mL, 6 × 15 mL
* Max. speed: 25000 ×g (15350 rpm)
* Acceleration/braking time: <25/25 s
* Temperature range: -11 °C to +40 °C
* Run time: 30 s to 99:59 h, infinity
[https://www.eppendorf.com/product-media/doc/en/335227/Centrifugation_Operating-manual_Centrifuge-5430-R.pdf Instruction manual] ''- requires login'' [[File:IMG_9457_Centrifuge 5430R.jpg|400px|thumb|left|Eppendorf centrifuge 5430R Location at DTU Nanolab Building 307 Room 903]]<br clear="all" />
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].


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[https://labmanager.dtu.dk/view_binary.php?class=MiscDocument&id=15&name=Eppendorf_Mini_Spin_plus_centrifuge_Original_Instructions.pdf Instruction manual] ''- requires login'' <br />
[https://labmanager.dtu.dk/view_binary.php?class=MiscDocument&id=15&name=Eppendorf_Mini_Spin_plus_centrifuge_Original_Instructions.pdf Instruction manual] ''- requires login'' <br />[[File:Mini Spin centrifuge.jpg|400px|thumb|left|Eppendorf Mini Spin centrifuge Location at DTU Nanolab Building 307 Room 903]]<br clear="all" />
 
[[File:Mini Spin centrifuge.jpg|400px|thumb|left|Eppendorf Mini Spin centrifuge Location at DTU Nanolab Building 307 Room 903]]<br clear="all" />
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].


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*Can be used at 5 to 40 °C (80% relative humidity), in an incubator or cold room
*Can be used at 5 to 40 °C (80% relative humidity), in an incubator or cold room
*Speed range 100 to 2500 (min-1)
*Speed range 100 to 2500 (min-1)


[[File:myPlate magnetic stirrer.jpg|400px|thumb|left|myPlate magnetic stirrer Location at DTU Nanolab Building 307 Room 903]]<br clear="all" />
[[File:myPlate magnetic stirrer.jpg|400px|thumb|left|myPlate magnetic stirrer Location at DTU Nanolab Building 307 Room 903]]<br clear="all" />
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[[File:Magnetic hotplate stirrer.jpeg|400px|thumb|left|VWR Magnetic hotplate stirrer Location at DTU Nanolab Building 314 Room 040]]<br clear="all" />
[[File:Magnetic hotplate stirrer.jpeg|400px|thumb|left|VWR Magnetic hotplate stirrer Location at DTU Nanolab Building 307 Room 903]]<br clear="all" />
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].


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[[File:Ohaus Analytical Balance PX224.jpg|400px|thumb|left|Ohaus PX224 analytical balance Location at DTU Nanolab Building 307 Room 903]]<br clear="all" />
[[File:IMG_9463_Analytical balance.jpg|400px|thumb|left|Ohaus PX224 analytical balance Location at DTU Nanolab Building 307 Room 903]]<br clear="all" />
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].


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== pH meter ==
== Midi 40 CO2 Incubator ==
The pH meter allows for accurate pH measurements of chemicals or ideal when making buffers.
The incubator is suitable for the incubation of cell culture and operates at temperatures ranging from 5°C above ambient temperature to 50°C ±0.2°C.
 
Specifications:
* Temperature range is from ambient +5° to 50°C ±0.2°C
* Relative Humidity is <90% at 37°C
* CO2 Sensor is TC Sensor
* Capacity is 40L
 
 
[[File:Midi 40 CO2 Incubator.jpg|400px|thumb|left|Midi 40 CO2 Incubator Location at DTU Nanolab Building 307 Room 903]]<br clear="all" />
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
 
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== Ultrasonic cleaner ==
The GT SONIC ultrasonic cleaner can be used to remove dirt, grease, corrosion or cleaning electronic and small mechanical parts. Millions of tiny bubbles are created when ultrasonic sound is passed through liquid. These tiny bubbles combined with a huge pressure create incredible cleaning results.
 
 
Specifications:
* Temperature range is from 30°C to 80°C
* Timer can be set from 1 to 99 minutes
* Capacity is 2L




[[File:VWR pH meter.jpg|400px|thumb|left|VWR pH meter Location at DTU Nanolab Building 314 Room 040]]<br clear="all" />
Four modes are available on the intrument and are as follows:
* '''Degas mode''' - This mode is to be used after a fluid change to rapidly remove air from the fluid so it can provide maximum cleaning effectiveness from the start of the cleaning cycle.
 
* '''Delicate mode''' - The cleaner runs at half power which provides a less aggressive cleaning action for fragile/delicate items.
 
* '''Full-power mode''' - A powerful deep clean that will clean heavily contaminated items.
 
* '''Temperature and Timing mode''' - Allows the user to set both the fluid temperature and duration of the cleaning cycle. These parameters will change depending on the cleaning agent and item being cleaned.
 
 
 
[[File:Ultrasonic cleaner.jpg|400px|thumb|left|GT SONIC Ultrasonic cleaner Location at DTU Nanolab Building 307 Room 903]]<br clear="all" />
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].


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== Midi 40 CO2 Incubator ==
== Drying oven ==
The incubator is suitable for the incubation of cell culture and operates at temperatures ranging from 5°C above ambient temperature to 60°C ±0.2°C.
The TS9026 drying oven is of 26L capacity and is used for epoxy embedding experiments.
 
 
Specifications:
* Temperature range is from ambient to 250°C
* Fan speed is 0 to 10
* Capacity is 26L


[[File:Midi 40 CO2 Incubator.jpg|400px|thumb|left|Midi 40 CO2 Incubator Location at DTU Nanolab Building 307 Room 903]]<br clear="all" />
[[File:IMG 9634.jpg|400px|thumb|left|TS9026 Drying oven Location at DTU Nanolab Building 307 Room 903]]<br clear="all" />
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].


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