Jump to content

LabAdviser/314/Preparation 314-307/Soft-matter: Difference between revisions

← Older edit
VisualWikitext
Mktracy (talk | contribs)
NLAB-Employees-701, NLAB-LabmanagerAllUsers, NLAB-Nlab307-labadviser-users-34891
620 edits
Mmat (talk | contribs)
NLAB-Employees-701, NLAB-LabmanagerAllUsers, Administrators
1,957 edits
mNo edit summary
 
(93 intermediate revisions by 2 users not shown)
Line 2: Line 2:
'''Feedback to this page''': '''[mailto:labadviser@nanolab.dtu.dk?Subject=Feed%20back%20from%20page%20http://labadviser.nanolab.dtu.dk/index.php/LabAdviser/314/Preparation_314-307/Soft-matter click here]'''
'''Feedback to this page''': '''[mailto:labadviser@nanolab.dtu.dk?Subject=Feed%20back%20from%20page%20http://labadviser.nanolab.dtu.dk/index.php/LabAdviser/314/Preparation_314-307/Soft-matter click here]'''


''This section is written by DTU Nanolab internal if nothing else is stated.''
[[Category:314]]
[[Category:314]]
[[Category:314-Preparation]]
[[Category:314-Preparation]]
Line 14: Line 15:




The main steps of this technique is as follows:<br />
[https://labmanager.dtu.dk/view_binary.php?class=MiscDocument&id=15&name=Leica_EM_GP2_Operating_manual.pdf Operating manual] ''- requires login''<br />  
1. The sample is spread onto a glow discharged EM grid <br />
2. The liquid droplet is then blotted with filter paper until only a very thin film of fluid remains <br />
3. The grid is then rapidly plunged into a cryogen (usually liquid ethane) <br />
4. The grid is afterwards stored in a grid box submerged in liquid nitrogen until finally loaded into the cryo-electron microscope for imaging <br />  


 
[[File:IMG_9460_GP2.jpg|400px|left|thumb|Leica EM GP2 Location at DTU Nanolab Building 307 Room 909B]]<br clear="all" />
Operating manual: [[:File:Leica EM GP2 Operating manual.pdf]]<br />
 
[[File:Plunge Freezer (2).jpg|400px|left|thumb|Leica EM GP2 Location at DTU Nanolab Building 314 Room 014 (Tecnai T20 room)]]<br clear="all" />


For further information on the equipment usage or training contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].  
For further information on the equipment usage or training contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].  
Line 29: Line 23:
<br clear="all" />
<br clear="all" />


<!--= High Pressure Freezer =
= High Pressure Freezer =
== Leica EM ICE ('''COMING SOON''') ==
== Leica EM ICE ==
High pressure freezing is key for the study of intricate changes in fine structure or cellular dynamics.
High pressure freezing is key for the study of intricate changes in fine structure or cellular dynamics.
* Cryo-immobilize your aqueous samples under high pressure with a unique freezing principle and uncover secrets of the cellular process.
* Cryo-immobilize your aqueous samples under high pressure with a unique freezing principle and uncover secrets of the cellular process.
Line 46: Line 40:




[[File:HPF EM ICE.jpg|600px|left|thumb|Leica EM ICE Location at DTU Nanolab Building 307]]<br clear="all" />
[[File:HPF EM ICE.jpg|600px|left|thumb|Leica EM ICE Location at DTU Nanolab Building 307 Room 909B]]<br clear="all" />
For further information on the equipment usage or training contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
For further information on the equipment usage or training contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].


Line 52: Line 46:


= Freeze substitution =
= Freeze substitution =
== Leica EM AFS2 and EM FSP ('''COMING SOON''') ==
== Leica EM AFS2 and EM FSP ==
The Leica EM AFS2 performs freeze substitution and progressive lowering of temperature (PLT) techniques and allows low temperature embedding and polymerization of resins. The Leica EM FSP (freeze substitution processor), an automatic reagent handling system combined with the Leica EM AFS2, dispenses reagents for both freeze substitution and PLT applications. The LED illumination from within the chamber and the attached stereomicroscope for viewing and positioning of samples ensures ease of use.
The Leica EM AFS2 performs freeze substitution and progressive lowering of temperature (PLT) techniques and allows low temperature embedding and polymerization of resins. The Leica EM FSP (freeze substitution processor), an automatic reagent handling system combined with the Leica EM AFS2, dispenses reagents for both freeze substitution and PLT applications. The LED illumination from within the chamber and the attached stereomicroscope for viewing and positioning of samples ensures ease of use.




[[File:Freeze-substitution EM AFS2.jpg|300px|left|thumb|Leica EM AFS2 Location at DTU Nanolab Building 307]]
[[File:Freeze-substitution EM AFS2.jpg|300px|left|thumb|Leica EM AFS2 Location at DTU Nanolab Building 307 Room 909B]]
[[File:EM FSP.JPG|200px|left|thumb|Leica EM FSP Location at DTU Nanolab Building 307]]<br clear="all" />
[[File:EM FSP.JPG|200px|left|thumb|Leica EM FSP Location at DTU Nanolab Building 307 Room 909B]]<br clear="all" />
For further information on the equipment usage or training contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
For further information on the equipment usage or training contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].


<br clear="all" /> -->
<br clear="all" />


= Microtome =
= Microtome =
== RMC MT-7 Microtome ==
Our RMC MT-7 Microtome is a vintage piece of equipment and can be used for specimen sample preparation for SEM/EDX analysis and/or for cutting relative thin slices (approx. 200nm thick) for TEM analysis. First, the specimen needs to be embedded into a resin or an epoxy, and then can be sectioned using a glass or diamond knife. 
[[File:RMC microtome.jpg|400px|thumb|left|RMC MT-7 Microtome Location at DTU Nanolab Building 307 Room 906]]<br clear="all" />
For further information about the microtome usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
<br clear="all" />
== Leica EM UC7 Ultramicrotome==
== Leica EM UC7 Ultramicrotome==
The Ultramicrotome Leica EM UC7 provides easy sample preparation of ultrathin sections for TEM, SEM, AFM and LM application. It is suitable for highly skilled or absolute beginners. The Leica ultramicrotome can provide ultra thin sectioning of sample embedded in a resin/epoxy block with a feed range of 1 nm up to 15 µm. Glass or diamond knife can be used for the ultra sectioning of the samples.  
The Ultramicrotome Leica EM UC7 provides easy sample preparation of ultrathin sections for TEM, SEM, AFM and LM application. It is suitable for highly skilled or absolute beginners. The Leica ultramicrotome can provide ultra thin sectioning of sample embedded in a resin/epoxy block with a feed range of 1 nm up to 15 µm. Glass or diamond knife can be used for the ultra sectioning of the samples.  




The main steps of this technique proceed as follows:<br />
[https://labmanager.dtu.dk/view_binary.php?class=MiscDocument&id=15&name=EM_UC7_Operating_manual.pdf Operating manual] ''-requires login''<br />
1. Mount sample <br />
2. Mount glass or diamond knife to knife holder <br />
3. Trimming or ultrasectioning of sample <br />
4. Fishing sections <br />
5. Transfer to an EM grid <br />


[[File:IMG_9461_UC7.jpg|400px|left|thumb|Leica EM UC7 Location at DTU Nanolab Building 307 Room 906]]<br clear="all" />


Operating manual: [[:File:EM UC7 Operating manual.pdf]]<br />
'''Requirement for training:'''
* Completion of the DTU Epoxy course
* Purchased of own diamond knife.  


[[File:Leica EM UC7.jpg|400px|left|thumb|Leica EM UC7 Location at DTU Nanolab Building 307 Room 906]]<br clear="all" />


'''Requirement for training:''' Completion of the DTU Epoxy course and purchased of own diamond knife. For further information about the equipment usage or training contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
For further information about the equipment usage or training contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].


<br clear="all" />
<br clear="all" />
Line 98: Line 78:




The main steps of this technique proceed as follows:<br />
[https://labmanager.dtu.dk/view_binary.php?class=MiscDocument&id=15&name=EM_FC7_Operating_manual.pdf Operating manual] ''- requires login''<br />  
1. Set up the cryo chamber EM FC7 to the EM UC7 apparatus <br />
2. Mount both the trim and sectioning diamond knives to the knive holder and set up in the cryo chamber <br />
3. Connect the pump tube between the liquid nitrogen Dewar and the ultramicrotome apparatus <br />
4. Fill up liquid nitrogen Dewar <br />
5. Let equipment to cool down for 1 hour <br />
6. Meanwhile prepare samples (Cut big samples to smaller pieces) <br />
7. Freeze sample in liquid nitrogen and mount sample <br />
8. Trimming and ultrasectioning of sample <br />
9. Collecting sections and transfer to an EM grid <br />


[[File:Leica EM FC7 Cryo-Ultramicrotome.jpg|400px|left|thumb|Leica EM FC7 Location at DTU Nanolab Building 307 Room 906]]<br clear="all" />


Operating manual: [[:File:EM FC7 Operating manual.pdf]]<br />
'''Requirement for training:'''
* Purchased of own diamond knife.  


[[File:Leica EM FC7 Cryo-Ultramicrotome.jpg|400px|left|thumb|Leica EM FC7 Location at DTU Nanolab Building 307 Room 906]]<br clear="all" />


'''Requirement for training:''' Purchased of own diamond knife. For further information about the equipment usage or training contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
For further information about the equipment usage or training contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].


<br clear="all" />
<br clear="all" />


= KnifeMaker=
= KnifeMaker=
== LKB Knifemaker 7801A ==
== Leica EM KMR3 ==
At DTU Nanolab we offer the possibility to make glass knives for ultramicrotomy applications using the LKB Knifemaker 7801A.
At DTU Nanolab we offer the possibility to make glass knives for ultramicrotomy applications using the Leica EM KMR3 Knifemaker.




Manual of LKB Knifemaker 7801A: [[:File:LKB Knifemaker 7800B Manual.pdf]]


[[File:Knifemaker.jpg|300px|thumb|left|LKB Knifemaker Location at DTU Nanolab Building 307 Room 906]]<br clear="all" />
[[File:IMG_9462_KMR3.jpg|400px|thumb|left|LKB Knifemaker Location at DTU Nanolab Building 307 Room 909B]]<br clear="all" />
For further information about the knifemaker usage and training contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
For further information about the knifemaker usage and training contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].


Line 140: Line 111:




Operating manual: [[:File:EM CPD300 Operating manual.pdf]] <br />  
[https://labmanager.dtu.dk/view_binary.php?class=MiscDocument&id=15&name=EM_CPD300_Operating_manual_small.pdf Operating manual] ''- requires login'' <br />  


[[File:EM CPD300.jpg|300px|thumb|left|Leica EM CPD300 Location at DTU Nanolab Building 314 Room 040]]<br clear="all" />
[[File:IMG_9456_CPD300.jpg|400px|thumb|left|Leica EM CPD300 Location at DTU Nanolab Building 307 Room 903]]<br clear="all" />
For further information on the CPD usage or training contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
For further information on the CPD usage or training contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].


<br clear="all" />
<br clear="all" />


= Drying oven =
= Coater =
The TS9026 drying oven is of 26L capacity and is used for epoxy embedding experiments.
== Leica EM ACE600 ==
 
The coater offers 3 applications
 
* Platinum coating
Specifications:
* Glow discharge
* Temperature range is from 2°C to 250°C
* Carbon coating
* Fan speed is 0 to 10
* Capacity is 26L
 
[[File:IMG 9634.jpg|400px|thumb|left|TS9026 Drying oven Location at DTU Nanolab Building 314 Room 040]]<br clear="all" />
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].


<br clear="all" />


= Sonic Dismembrator =
[[File:IMG_9459_ACE600.jpg|400px|thumb|left|Leica EM ACE600 Location at DTU Nanolab Building 307 Room 907]]<br clear="all" />
The Fisherbrand Model 505 sonic dismembrator apparatus applies sound energy to agitate particles in a sample. For biological applications, sonication may be sufficient to disrupt or deactivate a biological material. For example, sonication is often used to disrupt cell membranes and release cellular contents. In nanotechnology sonication is commonly used for evenly dispersing nanoparticles in liquids.
For further information about the coater usage and training contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
 
[[File:IMG_9633.jpg|400px|thumb|left|Fisherbrand Model 505 Sonic Dismembrator Location at DTU Nanolab Building 314 Room 040]]<br clear="all" />
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].


<br clear="all" />
<br clear="all" />
Line 182: Line 144:
* Cold room and incubator compatible
* Cold room and incubator compatible


[[File:IMG_9636.jpg|400px|thumb|left|Mini tube rotator Location at DTU Nanolab Building 314 Room 040]]<br clear="all" />
[[File:IMG_9636.jpg|400px|thumb|left|Mini tube rotator Location at DTU Nanolab Building 307 Room 903]]<br clear="all" />
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].


<br clear="all" />
<br clear="all" />


== Centrifuge ==
The Eppendorf Mini Spin plus centrifuge is a desktop centrifuge with maximum speed/force at 13,400rpm/12,100xG. It has a 30-minute timer, which is settable in 15-sec increments and has a short-spin mode fixed at maximum speed.


== Centrifuge 5430R ==
Two rotor are available for this centrifuge in the Softmatter lab.
* Rotor FA-45-30-11
Allows max. capacity 30 micro test tubes for 1.5/
2.0 mL and operates at 25000 ×g /15350 rpm
* Rotor F-35-6-30
Allows max. capacity 6 conical tubes for 50 mL or 6 conical tubes for 15 mL and operates at 7745 ×g /7830 rpm
'''Specifications:'''
'''Specifications:'''
* Max. rotor capacity depending on type of rotor: 30 × 1.5/2.0 mL, 6 × 50 mL, 6 × 15 mL
* Max. speed: 25000 ×g (15350 rpm)
* Acceleration/braking time: <25/25 s
* Temperature range: -11 °C to +40 °C
* Run time: 30 s to 99:59 h, infinity
[https://www.eppendorf.com/product-media/doc/en/335227/Centrifugation_Operating-manual_Centrifuge-5430-R.pdf Instruction manual] ''- requires login'' [[File:IMG_9457_Centrifuge 5430R.jpg|400px|thumb|left|Eppendorf centrifuge 5430R Location at DTU Nanolab Building 307 Room 903]]<br clear="all" />
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
<br clear="all" />
== Mini Centrifuge ==
The Eppendorf Mini Spin plus centrifuge is a desktop centrifuge and has a user friendly digital display for time and speed.
Specifications:
* Max. capacity 12 x 1.5/2.0 mL
* Max. RCF 14 100 g
* Speed 800 - 14 500 rpm (100 rpm steps)
* Acceleration time 13 s
* Timer 15 s to 99 mins


Instruction manual: [[:File:Eppendorf Mini Spin plus centrifuge_Original Instructions.pdf]]


[[File:Mini Spin centrifuge.jpg|400px|thumb|left|Eppendorf Mini Spin centrifuge Location at DTU Nanolab Building 314 Room 040]]<br clear="all" />
[https://labmanager.dtu.dk/view_binary.php?class=MiscDocument&id=15&name=Eppendorf_Mini_Spin_plus_centrifuge_Original_Instructions.pdf Instruction manual] ''- requires login'' <br />[[File:Mini Spin centrifuge.jpg|400px|thumb|left|Eppendorf Mini Spin centrifuge Location at DTU Nanolab Building 307 Room 903]]<br clear="all" />
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].


Line 201: Line 193:
== Magnetic stirrer ==
== Magnetic stirrer ==


The myPlate magnetic stirrer is ideal for homogeneous mixing chemicals. The speed range (min-1) is 100 - 2500.
The myPlate magnetic stirrer is ideal for homogeneous mixing of chemicals.




Line 207: Line 199:
*High chemical resistance
*High chemical resistance
*Can be used at 5 to 40 °C (80% relative humidity), in an incubator or cold room
*Can be used at 5 to 40 °C (80% relative humidity), in an incubator or cold room
*Speed range (min-1) 100 to 2500
*Speed range 100 to 2500 (min-1)


[[File:myPlate magnetic stirrer.jpg|400px|thumb|left|myPlate magnetic stirrer Location at DTU Nanolab Building 314 Room 040]]<br clear="all" />
 
[[File:myPlate magnetic stirrer.jpg|400px|thumb|left|myPlate magnetic stirrer Location at DTU Nanolab Building 307 Room 903]]<br clear="all" />
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].


Line 216: Line 209:
== Pulsing Vortex Mixer ==
== Pulsing Vortex Mixer ==


The VWR pulsing vortex mixer can suit different applications. It provides optimum comfort and minimal stress when used for the several applications.
The VWR pulsing vortex mixer provides ideal mixing of solutions or chemicals.




Line 224: Line 217:
* Have timer from 1 s to 160 h
* Have timer from 1 s to 160 h


Instruction manual: [[:File:VWR pulsing vortex mixer_Instruction Manual.pdf]]


[[File:IMG_9635.jpg|400px|thumb|left|VWR Pulsing Vortex Mixer Location at DTU Nanolab Building 314 Room 040]]<br clear="all" />
[https://labmanager.dtu.dk/view_binary.php?class=MiscDocument&id=15&name=VWR_pulsing_vortex_mixer_Instruction_Manual.pdf Instruction manual] ''- requires login'' <br />
 
[[File:IMG_9635.jpg|400px|thumb|left|VWR Pulsing Vortex Mixer Location at DTU Nanolab Building 307 Room 903]]<br clear="all" />
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].


Line 233: Line 227:
== Magnetic hotplate stirrer ==
== Magnetic hotplate stirrer ==


VWR Professional hotplate stirrers are designed for applications that require exceptional accuracy, stability and repeatability. Enhanced microprocessor control offers an external resistance thermometer (RTD) probe option that delivers superior temperature control of the sample. Stirring function, with continuous duty motor and powerful magnet, maintains set speed even under changing load or viscosity. Touch pad controls with easy to read, independent LED displays for temperature, speed and time, allow operator to view all settings at once. Rear housing features a built-in support rod holder with locking knob that accepts the supplied probe kit.
VWR Professional hotplate stirrer is ideal for stirring chemicals with controlled high temperature. An external resistance thermometer (RTD) probe option is available for temperature control of the sample.




Line 245: Line 239:




Low profile design takes up less space and fits into fume hoods. Spill resistant housing channels fluids away from internal components. A 'hot' symbol warning light is illuminated when heat is turned on and remains on until top plate cools down. Stir protection; if stirrer motor stops or fails, unit will automatically shut down heater. Plate over-temperature limit ensures plate temperature will never exceed users programmed set temperature limit, allowing for control of sensitive flash points. An audible alarm will sound when time reaches zero or when unit reaches set point temperature in timed mode, and if the probe disengages from sample, unit will automatically shut off heater. Microprocessor control with enhanced electronics regulates both heating and stirring and brings samples to temperature quickly and efficiently. Ramping feature slowly increases speed for improved safety and enhanced coupling. Avoids splashing, improves spin bar control and provides excellent low end speed control. Durable aluminium tops will not crack or chip, and provide a more even heating surface.
[[File:Magnetic hotplate stirrer.jpeg|400px|thumb|left|VWR Magnetic hotplate stirrer Location at DTU Nanolab Building 307 Room 903]]<br clear="all" />
 
[[File:Magnetic hotplate stirrer.jpeg|400px|thumb|left|VWR Magnetic hotplate stirrer Location at DTU Nanolab Building 314 Room 040]]<br clear="all" />
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].


Line 254: Line 246:
== Hotplate ==
== Hotplate ==


VWR Hotplate W10 is suitable for drying paraffin sections on slides or for de-icing tools when doing cryo experiments. The W10 heating plate, with its sleek compact design, accommodates two rows of up to 40 slides in total. The heating plate is very useful as a stretch bench for mounting, stretching and drying paraffin sections on slides, fixing bacterial smears, and heating fluid samples in Petri dishes. The black anodised surface provides an excellent visual contrast. The working plate is made of solid 8 mm aluminium with black anodised surface and ensures a constant, homogenous temperature over the entire heating surface. The touch display and switch are protected from water and paraffin. The unit has over-temperature protection for extra safety. Temperature adjustment and power On/Off switch, with control light for 'On' mode, are conveniently located at the front of the unit.
VWR Hotplate W10 is suitable for drying paraffin sections on microscope slides or for de-icing tools when doing cryo experiments.


 
Specifications:
*Electronic touch display
*Electronic touch display
*Sleek and compact   
*Sleek and compact   
*Ideal size for drying up to 40 slides
*Ideal size for drying up to 40 microscope slides
*Temperature range between ambient and +89 °C
*Temperature range between ambient and +89 °C
*Digital thermostat
*Digital thermostat




Instruction manual: [[:File:VWR Hotplate W10_Instruction Manual.pdf]]
[https://labmanager.dtu.dk/view_binary.php?class=MiscDocument&id=15&name=VWR_Hotplate_W10_Instruction_Manual.pdf Instruction manual] ''- requires login'' <br />




[[File:VWR Hotplate W10.jpg|400px|thumb|left|VWR Hotplate W10 Location at DTU Nanolab Building 314 Room 040]]<br clear="all" />
[[File:VWR Hotplate W10.jpg|400px|thumb|left|VWR Hotplate W10 Location at DTU Nanolab Building 307 Room 903]]<br clear="all" />
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].


Line 274: Line 267:
== Analytical balance PX224 ==
== Analytical balance PX224 ==


The Ohaus Pioneer PX224 combines essential weighing functionality with performance, offering high accuracy and repeatability for applications in laboratory, industrial and education settings. PX models are economically priced, and intuitively designed for intelligent operation. Their durable design has a cast metal lower housing, metal sub-pan and stainless steel weighing pan for long-term use.
The Ohaus Pioneer PX224 offers high accuracy and repeatability weight measurements.




Specifications:
*Weighing capacity is 220 g
*Weighing capacity is 220 g
*Readability is 0.1 mg
*Readability is 0.1 mg
Line 285: Line 279:




[[File:Ohaus Analytical Balance PX224.jpg|400px|thumb|left|Ohaus PX224 analytical balance Location at DTU Nanolab Building 314 Room 040]]<br clear="all" />
[[File:IMG_9463_Analytical balance.jpg|400px|thumb|left|Ohaus PX224 analytical balance Location at DTU Nanolab Building 307 Room 903]]<br clear="all" />
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
 
<br clear="all" />
 
== Midi 40 CO2 Incubator ==
The incubator is suitable for the incubation of cell culture and operates at temperatures ranging from 5°C above ambient temperature to 50°C ±0.2°C.
 
Specifications:
* Temperature range is from ambient +5° to 50°C ±0.2°C
* Relative Humidity is <90% at 37°C
* CO2 Sensor is TC Sensor
* Capacity is 40L
 
 
[[File:Midi 40 CO2 Incubator.jpg|400px|thumb|left|Midi 40 CO2 Incubator Location at DTU Nanolab Building 307 Room 903]]<br clear="all" />
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
 
<br clear="all" />
 
== Ultrasonic cleaner ==
The GT SONIC ultrasonic cleaner can be used to remove dirt, grease, corrosion or cleaning electronic and small mechanical parts. Millions of tiny bubbles are created when ultrasonic sound is passed through liquid. These tiny bubbles combined with a huge pressure create incredible cleaning results.
 
 
Specifications:
* Temperature range is from 30°C to 80°C
* Timer can be set from 1 to 99 minutes
* Capacity is 2L
 
 
Four modes are available on the intrument and are as follows:
* '''Degas mode''' - This mode is to be used after a fluid change to rapidly remove air from the fluid so it can provide maximum cleaning effectiveness from the start of the cleaning cycle.
 
* '''Delicate mode''' - The cleaner runs at half power which provides a less aggressive cleaning action for fragile/delicate items.
 
* '''Full-power mode''' - A powerful deep clean that will clean heavily contaminated items.
 
* '''Temperature and Timing mode''' - Allows the user to set both the fluid temperature and duration of the cleaning cycle. These parameters will change depending on the cleaning agent and item being cleaned.
 
 
 
[[File:Ultrasonic cleaner.jpg|400px|thumb|left|GT SONIC Ultrasonic cleaner Location at DTU Nanolab Building 307 Room 903]]<br clear="all" />
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].


<br clear="all" />
<br clear="all" />


== pH meter ==
== Drying oven ==
If you are planning on doing chemical fixation on biological samples you will probably need to make buffers and use a pH-meter. The VWR pH-meter (model pH 1000L) is located in building 314, in the Soft Mater (toxic) Lab and it is dedicated to “Soft Matter” related work.
The TS9026 drying oven is of 26L capacity and is used for epoxy embedding experiments.
 


Specifications:
* Temperature range is from ambient to 250°C
* Fan speed is 0 to 10
* Capacity is 26L


[[File:VWR pH meter.jpg|400px|thumb|left|VWR pH meter Location at DTU Nanolab Building 314 Room 040]]<br clear="all" />
[[File:IMG 9634.jpg|400px|thumb|left|TS9026 Drying oven Location at DTU Nanolab Building 307 Room 903]]<br clear="all" />
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
For further information on the equipment usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].


Retrieved from "https://labadviser.nanolab.dtu.dk//index.php?title=LabAdviser/314/Preparation_314-307/Soft-matter"