Jump to content

LabAdviser/314/Preparation 314-307/Soft-matter/Ultramicrotomy

From LabAdviser

Feedback to this page: click here (content by Marie Karen Tracy Hong Lin @DTU Nanolab, 2020)

Procedure of ultra-sectionning

Step 1: Trimming resin block

 
1. Trimming resin block using Trim diamond knife



Step 2: Mount sample to the ultramicrotome


Step 3: Mount glass or diamound knife to knife holder


Step 4: Ultra-sectioning of sample

 
4. Ultra-sectionning of sample using diamond knife with water boat



Step 5: Fishing sections

 
5. Collecting sections using a Perfect loop



Step 6: Transfering sections to EM grid

 
6. Transfering sections on a grid