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== Leica EM FC7 Cryo-Ultramicrotome ==
== Leica EM FC7 Cryo-Ultramicrotome ==
Within minutes the Leica EM UC7 ultramicrotome can be change to a cryo-ultramicrotome by mounting the cryo chamber EM FC7. Using the cryo-ultramicrotome, cryo-sections (-15° to -185°C) can be prepared for TEM, SEM, AFM and LM applications.
Within minutes the Leica EM UC7 ultramicrotome can be change to a cryo-ultramicrotome by mounting the cryo chamber EM FC7. Using the cryo-ultramicrotome, cryo-sections (-15° to -185°C) can be prepared for TEM, SEM, AFM and LM applications.
The main steps of this technique proceed as follows:<br />
1. Set up the cryo chamber EM FC7 to the EM UC7 apparatus <br />
2. Mount both the trim and sectioning diamond knives to the knive holder and set up in the cryo chamber <br />
3. Connect the pump tube between the liquid nitrogen Dewar and the ultramicrotome apparatus <br />
4. Fill up liquid nitrogen Dewar <br />
5. Let equipment to cool down for 1 hour <br />
6. Meanwhile prepare samples (Cut big samples to smaller pieces) <br />
7. Freeze sample in liquid nitrogen and mount sample <br />
8. Trimming and ultrasectioning of sample <br />
9. Collecting sections and transfer to an EM grid <br />




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