Jump to content

LabAdviser/314/Preparation 314-307/Soft-matter: Difference between revisions

Mktracy (talk | contribs)
Mktracy (talk | contribs)
Line 66: Line 66:
== Leica EM UC7 Ultramicrotome==
== Leica EM UC7 Ultramicrotome==
The Ultramicrotome Leica EM UC7 provides easy sample preparation of ultrathin sections for TEM, SEM, AFM and LM application. It is suitable for highly skilled or absolute beginners. The Leica ultramicrotome can provide ultra thin sectioning of sample embedded in a resin/epoxy block with a feed range of 1 nm up to 15 µm. Glass or diamond knife can be used for the ultra sectioning of the samples.  
The Ultramicrotome Leica EM UC7 provides easy sample preparation of ultrathin sections for TEM, SEM, AFM and LM application. It is suitable for highly skilled or absolute beginners. The Leica ultramicrotome can provide ultra thin sectioning of sample embedded in a resin/epoxy block with a feed range of 1 nm up to 15 µm. Glass or diamond knife can be used for the ultra sectioning of the samples.  
The main steps of this technique proceed as follows:<br />
1. Mount sample <br />
2. Mount glass or diamond knife to knife holder <br />
3. Trimming or ultrasectioning of sample <br />
4. Fishing sections <br />
5. Transfer to an EM grid <br />