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LabAdviser/314/Preparation 314-307/Soft-matter: Difference between revisions

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== Leica EM CPD300 ==
== Leica EM CPD300 ==


The CPD is used to preserve the sample’s morphology. Since air drying would cause severe deformation and collapse of structure due to the effects of high surface tension to air, the water in the biological specimen is replaced with a suitable inert fluid (“transitional fluid”-CO2) which has a lower surface tension to air. This would thus reduce severe structural damages during air-drying.  
The CPD allows for ideal preservation of specimen's microstructures during drying processes prior to SEM analysis. Since air drying could cause severe deformation and collapse of microstructure due to the effects of high surface tension to air, the water in the biological specimen is replaced with a suitable inert fluid (“transitional fluid”-CO2) which has a lower surface tension to air. This would thus reduce severe structural damages during drying processes.