LabAdviser/314/Microscopy 314-307/TEM/ETEM/OneView: Difference between revisions
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[[image:Gatan_OneView_1.png|400x400px|right|thumb|GMS3 main window.]] | [[image:Gatan_OneView_1.png|400x400px|right|thumb|GMS3 main window.]] | ||
1. If | 1. If the computer for the OneView camera is not running (large screen), start the computer. It is located in the technical room behind the microscope (large computer with a lot of hard drive slots). The login is:<br /> | ||
: user: '''Administrator'''<br /> | |||
: PW: '''$admin''' | |||
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2. | 2. If GMS3 (Gatan Microscopy Suite; also known as Digital Micrograph) is not running yet, click on the GMS icon and acknowledge any information message (elevated privileges; using potentially harmful plug-ins).<br /> | ||
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3. | 3. Check in the '''Camera Monitor''' that the temperature is -5°C. Else, wait until it reaches this temperature.<br /> | ||
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4. | 4. On the Microscope PC, the software '''Gatan Remote''' should be running in the background. If not, start this software.<br /> | ||
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5. On the left hand side under '''Microscope''', GMS shows the visualization of the TEM. Here, HT and Magnification should be the same as in the Microscope PC. Clicking on '''Valves''' will open or close the Column Valves, '''Blank''' will blank the beam before the sample, clicking on the '''Phosphorous screen''' will lift or lower the screen. Right click on the '''OneView''' camera icon opens a context menu, where the camera can be inserted or retracted.<br /><br /> | 5. Make sure you are an '''Power User'''. Click on '''Help''' in the top task bar and select '''Power User''' under '''User Mode'''. Now you have all relevant settings and properties available.<br /> | ||
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6. On the left hand side under '''Microscope''', GMS shows the visualization of the TEM. Here, HT and Magnification should be the same as in the Microscope PC. Clicking on '''Valves''' will open or close the Column Valves, '''Blank''' will blank the beam before the sample, clicking on the '''Phosphorous screen''' will lift or lower the screen. Right click on the '''OneView''' camera icon opens a context menu, where the camera can be inserted or retracted.<br /><br /> | |||
!!! The Ultrascan camera needs to be retracted before inserting the OneView camera !!!<br /> | !!! The Ultrascan camera needs to be retracted before inserting the OneView camera !!!<br /> | ||
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7. On the frontpage right hand side, you find the '''Technique Manager'''. Under '''TEM''' you find '''TEM imaging''' and '''In-situ imaging'''.<br /> | |||
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* Each frame will be saved hierarchically in the folder under '''Hour_xx''', '''Minute_xx''', '''Second_xx'''.<br /> | * Each frame will be saved hierarchically in the folder under '''Hour_xx''', '''Minute_xx''', '''Second_xx'''.<br /> | ||
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== Additional information == | == Additional information == | ||