LabAdviser/314/Microscopy 314-307/SEM/QFEG: Difference between revisions
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= Examples of the QFEG's capabilities = | = Examples of the QFEG's capabilities = | ||
== SEM (Scanning Electron Microscopy) == | |||
The SEM is a remarkable technique which can offer information such as the surface morphology of a sample and also a 3D appearance of the specimen. It can offer the best resolution (2 nm at 30kV) at high magnification and also topographical details because of its great depth of field. | The SEM is a remarkable technique which can offer information such as the surface morphology of a sample and also a 3D appearance of the specimen. It can offer the best resolution (2 nm at 30kV) at high magnification and also topographical details because of its great depth of field. | ||
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== BSD (Backscattered Electron Detector) == | |||
[[File:20201017_Microalgae_Image01_019-1.jpg|500px|left|thumb|BSD imaging of Microalgae with gold nanoparticles]]<br clear="all" /> | [[File:20201017_Microalgae_Image01_019-1.jpg|500px|left|thumb|BSD imaging of Microalgae with gold nanoparticles]]<br clear="all" /> | ||
== STEM (Scanning Transmission Electron Microscopy) == | |||
[[File:20210519_Microalgae_K2_007-1.jpg|500px|left|thumb|STEM imaging of a cross section of Microalgae]]<br clear="all" /> | [[File:20210519_Microalgae_K2_007-1.jpg|500px|left|thumb|STEM imaging of a cross section of Microalgae]]<br clear="all" /> | ||
[[File:20210413_Yeast_S1_005-1.jpg|500px|left|thumb|STEM imaging of a cross section of Yeast]]<br clear="all" /> | [[File:20210413_Yeast_S1_005-1.jpg|500px|left|thumb|STEM imaging of a cross section of Yeast]]<br clear="all" /> | ||
== ESEM (Environmental Scanning Electron Microscopy) == | |||
In the ESEM setting, sample temperature and specimen chamber vapour pressure can both be controlled thus allowing visualiztion of the specimen when heated, cooled, wetted or dried. | In the ESEM setting, sample temperature and specimen chamber vapour pressure can both be controlled thus allowing visualiztion of the specimen when heated, cooled, wetted or dried. | ||
== Cryo-SEM == | |||
In the cryo-SEM setting, the sample is visualized in a frozen state (down to -190 °C). This setting is very useful when the specimen being observed needs visualiztion in its hydrated form. It can be imaged by using either the secondary electrons (SE) or backscattered electrons (BSE) detector. | In the cryo-SEM setting, the sample is visualized in a frozen state (down to -190 °C). This setting is very useful when the specimen being observed needs visualiztion in its hydrated form. It can be imaged by using either the secondary electrons (SE) or backscattered electrons (BSE) detector. | ||