LabAdviser/314/Microscopy 314-307/TEM/ETEM/OneView: Difference between revisions

Revision as of 12:23, 21 September 2021

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Gatan OneView camera and GMS3

Here are a few information on the OneView camera and the control in GMS3:

General info

- CMOS camera with 4096 x 4096px (pixel size: 15µm) --> 61.4 x 61.4mm sensor size; conversion efficiency @300kV: 26.42 ADC counts/Prim e- (our camera)
- Imaging mode: 25fps for full 4k x 4k resolution (single frame exposure time: 0.04s); 100fps @ 2k x 2k; 200fps @ 1k x 1k; 300fps @ 512 x 512
- Diffraction mode: about 7fps for full 4k x 4k (single frame exposure time: 0.16s)
- drift correction available
- In in situ mode: look back option available up to 20s

Operation

image acquisition

1. If GMS3 (Gatan Microscopy Suite; also known as Digital Micrograph) is not running on the separate computer (large screen) yet, click on the GMS icon and acknowledge any information message.

2. Check in the Camera Monitor that the temperature is -5C. Else, wait until it reaches this temperature.

3. On the Microscope PC, the software Gatan Remote should be running in the background. If not, start this software.

4. On the left hand side under Microscope, GMS shows the visualization of the TEM. Here, HT and Magnification should be the same as in the Microscope PC. Clicking on Valves will open or close the Column Valves, Blank will blank the beam before the sample, clicking on the Phosphorous screen will lift or lower the screen. Right click on the OneView camera icon opens a context menu, where the camera can be inserted or retracted.

!!! The Ultrascan camera needs to be retracted before inserting the OneView camera !!!

Feedback to this page: click here

@@ Line 22: / Line 22: @@
 === image acquisition ===
-. If GMS3 (Gatan Microscopy Suite; also known as Digital Micrograph) is not running yet on the separate computer (large screen), click on the GMS icon and acknowledge any information message.<br />
+. If GMS3 (Gatan Microscopy Suite; also known as Digital Micrograph) is not running on the separate computer (large screen) yet, click on the GMS icon and acknowledge any information message.<br />
 <br />
-.
+. Check in the '''Camera Monitor''' that the temperature is -5C. Else, wait until it reaches this temperature.<br />
+<br />
+. On the Microscope PC, the software '''Gatan Remote''' should be running in the background. If not, start this software.<br />
+<br />
+. On the left hand side under '''Microscope''', GMS shows the visualization of the TEM. Here, HT and Magnification should be the same as in the Microscope PC. Clicking on '''Valves''' will open or close the Column Valves, '''Blank''' will blank the beam before the sample, clicking on the '''Phosphorous screen''' will lift or lower the screen. Right click on the '''OneView''' camera icon opens a context menu, where the camera can be inserted or retracted.<br /><br />
+!!! The Ultrascan camera needs to be retracted before inserting the OneView camera !!!<br />
+<br /><br />
-Go to "Customs" and "SetDoseCalibrationTag_OneView_mod-DTU" <br />
-[[File:Oneview_1.png|600px|center|thumb|Run script]]
-<br clear="all" />
-. Type in the high tension the microscope is running in the pop-up window<br />
-[[File:Oneview_2.png|300px|center|thumb|Select high tension]]
-<br clear="all" />
-. Don't change the conversion factor; just click on "OK"<br />
+<!-- Go to "Customs" and "SetDoseCalibrationTag_OneView_mod-DTU" <br />
-[[File:Oneview_3.png|300px|center|thumb|Don't change conversion factor]]
+[[File:Oneview_1.png|600px|center|thumb|Run script]]
-<br clear="all" />
+<br clear="all" /> -->
 '''Feedback to this page''': '''[mailto:labadviser@nanolab.dtu.dk?Subject=Feed%20back%20from%20page%20http://labadviser.nanolab.dtu.dk/index.php/LabAdviser/314/ETEM/OneView click here]'''