LabAdviser/314/Microscopy 314-307/TEM/ETEM/OneView

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Gatan OneView camera and GMS3

Here are a few information on the OneView camera and the control in GMS3:

General info

- CMOS camera with 4096 x 4096px (pixel size: 15µm) --> 61.4 x 61.4mm sensor size; conversion efficiency @300kV: 26.42 ADC counts/Prim e- (our camera)
- Imaging mode: 25fps for full 4k x 4k resolution (single frame exposure time: 0.04s); 100fps @ 2k x 2k; 200fps @ 1k x 1k; 300fps @ 512 x 512
- Diffraction mode: about 7fps for full 4k x 4k (single frame exposure time: 0.16s)
- drift correction available
- In in situ mode: look back option available up to 20s

Operation

image acquisition

1. If GMS3 (Gatan Microscopy Suite; also known as Digital Micrograph) is not running yet on the separate computer (large screen), click on the GMS icon and acknowledge any information message.

2.

Go to "Customs" and "SetDoseCalibrationTag_OneView_mod-DTU"

Run script

2. Type in the high tension the microscope is running in the pop-up window

Select high tension

3. Don't change the conversion factor; just click on "OK"

Don't change conversion factor

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