LabAdviser/314/Preparation 314-307/Soft-matter/Ultramicrotomy: Difference between revisions

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== Step 6: Transfering sections ==
== Step 6: Transfering sections to EM grid ==
[[File:5 (2).jpg|400px|left|thumb|6. Transfering sections on a grid]]<br />
[[File:5 (2).jpg|400px|left|thumb|6. Transfering sections on a grid]]<br />

Revision as of 09:54, 8 July 2020

Procedure of ultra-sectionning

Step 1: Trimming resin block

1. Trimming resin block using Trim diamond knife



Step 2: Mount sample to the ultramicrotome


Step 3: Mount glass or diamound knife to knife holder


Step 4: Ultra-sectioning of sample

4. Ultra-sectionning of sample using diamond knife with water boat



Step 5: Fishing sections

5. Collecting sections using a Perfect loop



Step 6: Transfering sections to EM grid

6. Transfering sections on a grid


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