LabAdviser/314/Microscopy 314-307/Technique/Holo/Off-axis ATEM: Difference between revisions
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# Swap the cables at the SAD apertures (from grey to red) <br/><br/> | # Swap the cables at the SAD apertures (from grey to red) <br/><br/> | ||
# Switch to aperture #3. Remove the SAD apertures from the column by moving the lever from left to right. | # Switch to aperture #3. Remove the SAD apertures from the column by moving the lever from left to right. | ||
== Acquiring CBED patterns == | |||
# Decrease the magnification to SA 7200X or something in the SA range. <br/><br/> | |||
# Start the camera acquisition. Lift the viewing screen (press R1 button) <br/><br/> | |||
# Go to Mono tab → Monochromator tune → Click on focus button and play with the intensity knob by turning it clockwise and decrease the intensity drastically, say to the value of 100 or 175 or 200, the higher the better as there will be less damage to the sample. We want to have a beam that gives a CBED pattern that is not saturated under acquisition times of 0.001-0.01 s. This way you will get a beam that doesn’t burn the featyre of interest. Go to a magnification around SA 49000X. <br/><br/> | |||
# Go to Gun tab → Click on the TEM button <br/><br/> | |||
# Acquire CBED pattern after tilting the sample appropriately. Use an acquisition time of 0.001 sec or 0.01 sec. Use an acquisition time as low as possible otherwise you will burn the camera. | |||
== Closing down the session == | |||
# Stop the camera acquisition. <br/><br/> | |||
# Put the screen down (press R1 button) <br/><br/> | |||
# Reset the holder <br/><br/> | |||
# Make sure that all apertures (SAD and objective) are retracted. <br/><br/> | |||
# Close the column isolation valve. Go to Set up tab → Vacuum → Click on column valve closed button (the button’s color will change from grey to yellow) <br/><br/> | |||
# Remove the holder <br/><br/> | |||
# Fill out the process log <br/><br/> | |||
# Check on the lab manager whether there is a next user | |||