LabAdviser/314/Preparation 314-307/Soft-matter: Difference between revisions
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3. The grid is then rapidly plunged into a cryogen (usually liquid ethane) <br /> | 3. The grid is then rapidly plunged into a cryogen (usually liquid ethane) <br /> | ||
4. The grid is afterwards stored in a grid box submerged in liquid nitrogen until finally loaded into the cryo-electron microscope for imaging <br /> | 4. The grid is afterwards stored in a grid box submerged in liquid nitrogen until finally loaded into the cryo-electron microscope for imaging <br /> | ||
Details on the procedure is illustrated as follows: | |||
*[[/Plunge Freezing|Procedure steps]]<br /> | |||
Operating manual: [[:File:Leica EM GP2 Operating manual.pdf]]<br /> | Operating manual: [[:File:Leica EM GP2 Operating manual.pdf]]<br /> | ||
Risk Assessment: [[:File:Risk assessment - EM GP2 Plunge Freezer.pdf]]<br /> | Risk Assessment: [[:File:Risk assessment - EM GP2 Plunge Freezer.pdf]]<br /> | ||