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LabAdviser/314/Microscopy 314-307/Technique/Holo/Off-axis ETEM: Difference between revisions

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In order to remove effects from the TEM or other instabilities, reference holograms are usually acquired right after the object holograms. They are later used for reconstruction.
In order to remove effects from the TEM or other instabilities, reference holograms are usually acquired right after the object holograms. They are later used for reconstruction.


[[file:Schematic_holo.jpg|thumb|left|200px|Figure 1: Schematic of the set-up for off-axis electron holography. U0 is the biprism voltage, s is the fringe spacing, β is the angle that the object and reference wave is superimposed onto one another, and w is the width of the interference pattern.]] <br clear=all> <br/>
:[[file:Schematic_holo.jpg|thumb|left|200px|Figure 1: Schematic of the set-up for off-axis electron holography. U0 is the biprism voltage, s is the fringe spacing, β is the angle that the object and reference wave is superimposed onto one another, and w is the width of the interference pattern.]] <br clear=all>


The detailed procedure for obtaining electron holograms is described below. The procedure has been used for obtaining holograms of in liquid phase transmission electron microscopy or of single features on a C-grid. Some tips are also shown at the end of the document.
The detailed procedure for obtaining electron holograms is described below. The procedure has been used for obtaining holograms of in liquid phase transmission electron microscopy or of single features on a C-grid. Some tips are also shown at the end of the document.