LabAdviser/314/Microscopy 314-307/Technique/Holo/Off-axis ATEM: Difference between revisions

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 #	 Correct for objective lens astigmatism. For every magnification change you should recheck objective lens astigmatism and the focus (with the focus knob). You do the rotation centering at the beginning and not when you play with the magnification-control. <br/><br/>
 #	Acquire a reference hologram with acquisition time of 10 s and if it is noisy reacquire the hologram with 10 s or 5 s.  Then draw a spectrum line across a few fringes and see how many pixels are there per fringe. We should keep 4 pixels per fringe.  To increase the number of fringes per unit are, increase the biprism voltage. A biprism voltage of 120-130V is recommended.
+== Finishing-up the holography part of the measurement ==
+#	Stop the camera acquisition <br/><br/>
+#	Put the viewing screen down (with R1 button) <br/><br/>
+#	Come out of the Lorentz and Holography modes. To do this go to Holography tab &rarr; Click on Holography button again to unselect it. Then go to Lorentz &rarr; Click on Lorentz button again to unselect it. Then switch to the TEM mode (Gun &rarr; BeamSettings &rarr; Click on TEM button). <br/><br/>
+#	Turn down the volume knob of the biprism. Then turn off this box. <br/><br/>
+#	Turn off the power supply box in the back of the room. <br/><br/>
+#	Swap the cables at the SAD apertures (from grey to red) <br/><br/>
+#	Switch to aperture #3. Remove the SAD apertures from the column by moving the lever from left to right.