LabAdviser/314/Preparation 314-307/Soft-matter/Ultramicrotomy: Difference between revisions

From LabAdviser
Mktracy (talk | contribs)
No edit summary
Mktracy (talk | contribs)
No edit summary
Line 7: Line 7:
<br clear="all" />
<br clear="all" />


== Step 2: Ultra-sectioning of sample ==
== Step 2: Mount sample to the ultramicrotome ==
[[File:8 (2).jpg|400px|left|thumb|2. Ultra-sectionning of sample using diamond knife with water boat]]<br />


<br clear="all" />
<br clear="all" />


== Step 3: Collecting sections ==
== Step 3: Mount glass or diamound knife to knife holder ==
[[File:9 (2).jpg|400px|left|thumb|3. Collecting sections using a Perfect loop]]<br />


<br clear="all" />
<br clear="all" />


== Step 4: Transfering sections ==
== Step 4: Ultra-sectioning of sample ==
[[File:5 (2).jpg|400px|left|thumb|4. Transfering sections on a grid]]<br />
[[File:8 (2).jpg|400px|left|thumb|4. Ultra-sectionning of sample using diamond knife with water boat]]<br />
 
<br clear="all" />
 
== Step 5: Fishing sections ==
[[File:9 (2).jpg|400px|left|thumb|5. Collecting sections using a Perfect loop]]<br />
 
<br clear="all" />
 
== Step 6: Transfering sections ==
[[File:5 (2).jpg|400px|left|thumb|6. Transfering sections on a grid]]<br />

Revision as of 09:54, 8 July 2020

Procedure of ultra-sectionning

Step 1: Trimming resin block

1. Trimming resin block using Trim diamond knife



Step 2: Mount sample to the ultramicrotome


Step 3: Mount glass or diamound knife to knife holder


Step 4: Ultra-sectioning of sample

4. Ultra-sectionning of sample using diamond knife with water boat



Step 5: Fishing sections

5. Collecting sections using a Perfect loop



Step 6: Transfering sections

6. Transfering sections on a grid