LabAdviser/314/Preparation 314-307/Soft-matter: Difference between revisions
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== Leica EM GP2 == | == Leica EM GP2 == | ||
The | The plunge freezer is a cryo-fixation method used to preserve samples in their most native state prior to cryo-electron microscopy. | ||
The main steps of this technique proceed as follows:<br /> | |||
1. The sample is spread into a thin film across an EM grid <br /> | |||
2. The liquid droplet is then blotted with filter paper until only a very thin film of fluid remains <br /> | |||
3. The grid is then rapidly plunged into a cryogen (usually liquid ethane) <br /> | |||
4. The grid is afterwards stored in liquid nitrogen in grid box until finally loaded into the cryo-electron microscope for imaging <br /> | |||
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For further information on the machine usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0]. | For further information on the machine usage or training contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0]. | ||