LabAdviser/314/Preparation 314-307: Difference between revisions
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'''Leica EM CPD300.''' | '''Leica EM CPD300.''' | ||
The Critical Point Drier (CPD) is used for drying samples for the SEM. The machine uses the fact that at the critical point the solvent can be converted from liquid to gas without crossing the interfaces between liquid and gas (no surface tension) and hence no sample drying artefacts. CO2 is the solvent of choice as its triple point is suitable for biological samples (31°C and 74 bars). The CO2 is not miscible with water; therefore the sample should be dissolved in acetone or ethanol. | The Critical Point Drier (CPD) is used for drying samples for the SEM. The machine uses the fact that at the critical point the solvent can be converted from liquid to gas without crossing the interfaces between liquid and gas (no surface tension) and hence no sample drying artefacts. CO2 is the solvent of choice as its triple point is suitable for biological samples (31°C and 74 bars). The CO2 is not miscible with water; therefore the sample should be dissolved in acetone or ethanol. | ||
The Leica EM CPD300 operating manual is here [[:File:EM CPD300 Operating manual.pdf]] and the Risk Assessment here [[:File:Risk assessment-EM CPD300.docx]]. | The Leica EM CPD300 operating manual is here [[:File:EM CPD300 Operating manual.pdf]] and the Risk Assessment here [[:File:Risk assessment-EM CPD300.docx]]. | ||