LabAdviser/314/Microscopy 314-307/SEM/QFEG: Difference between revisions
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''This section is written by DTU Nanolab internal if nothing else is stated.'' | |||
[[Category:314]] | [[Category:314]] | ||
[[Category:314-Microscopy]] | [[Category:314-Microscopy]] | ||
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=QFEG 200 Cryo ESEM (FEI Quanta FEG 200)= | =QFEG 200 Cryo ESEM (FEI Quanta FEG 200)= | ||
[[ | [[File:202231020_IMG_4763.jpg|400px|right|thumb| DTU Nanolab QFEG located in building 307 room 907]] | ||
The QFEG 200 Cryo is an FEI Quanta FEG (Field Emission Gun) scanning electron microscope with a spatial resolution of 2 nm for the ETD detector in high vacuum at 30 keV. The microscope can operate in several modes, such as high vacuum, low vacuum, environmental mode, room temperature, low temperature and cryo. Our QFEG is fitted with an EDS detector, which allows for analytical measurements in all the operation modes. With the cryo transfer system available, it is especially suited for cryo experiments. | The QFEG 200 Cryo is an FEI Quanta FEG (Field Emission Gun) scanning electron microscope with a spatial resolution of 2 nm for the ETD detector in high vacuum at 30 keV. The microscope can operate in several modes, such as high vacuum, low vacuum, environmental mode, room temperature, low temperature and cryo. Our QFEG is fitted with an EDS detector, which allows for analytical measurements in all the operation modes. With the cryo transfer system available, it is especially suited for cryo experiments. | ||
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= Examples of the QFEG's capabilities = | = Examples of the QFEG's capabilities = | ||
''All images are taken by Marie Karen Tracy Hong Lin @DTU Nanolab'' | |||
== SEM (Scanning Electron Microscopy) == | == SEM (Scanning Electron Microscopy) == | ||
The SEM is a remarkable technique which can offer information such as the surface morphology of a sample and also a 3D appearance of the specimen. It can offer the best resolution (2 nm at 30kV) at high magnification and also topographical details because of its great depth of field. | The SEM is a remarkable technique which can offer information such as the surface morphology of a sample and also a 3D appearance of the specimen. It can offer the best resolution (2 nm at 30kV) at high magnification and also topographical details because of its great depth of field. | ||
[[File:20201017_Microalgae_Image01_018-1.jpg|500px|left|thumb|SEM imaging of Microalgae with gold nanoparticles]]<br clear="all" /> | [[File:20201017_Microalgae_Image01_018-1.jpg|500px|left|thumb|SEM imaging of Microalgae with gold nanoparticles]]<br clear="all" /> | ||
[[File:20191217 Bacteria Image_032-1.jpg|500px|left|thumb|SEM imaging of Bacteria]]<br clear="all" /> | [[File:20191217 Bacteria Image_032-1.jpg|500px|left|thumb|SEM imaging of Bacteria]]<br clear="all" /> | ||
[[File:Spider_006-1.jpg|500px|left|thumb|SEM imaging of Spider]]<br clear="all" /> | [[File:Spider_006-1.jpg|500px|left|thumb|SEM imaging of Spider]]<br clear="all" /> | ||
[[File:Spider Web_020-1.jpg|500px|left|thumb|SEM imaging of Spider web]]<br clear="all" /> | [[File:Spider Web_020-1.jpg|500px|left|thumb|SEM imaging of Spider web]]<br clear="all" /> | ||
== BSD (Backscattered Electron Detector) == | == BSD (Backscattered Electron Detector) == | ||
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== STEM (Scanning Transmission Electron Microscopy) == | == STEM (Scanning Transmission Electron Microscopy) == | ||
STEM combines the principles of transmission electron microscopy and scanning electron microscopy and can be performed on either type of instrument. In the STEM setting, the sample requires to have a thickness of less than 200 nm for visualization. Sample preparation prior to microscopy involve samples being embedded in epoxy resin and then ultra | STEM combines the principles of transmission electron microscopy and scanning electron microscopy and can be performed on either type of instrument. In the STEM setting, the sample requires to have a thickness of less than 200 nm for visualization. Sample preparation prior to STEM microscopy involve samples being embedded in epoxy resin and then ultra sectioning using the [[LabAdviser/314/Preparation_314-307/Soft-matter#Leica_EM_UC7_Ultramicrotome|Ultramicrotome]]. For more sensitive samples that need to be sectionned at cryogenic temperatures (below −180 °C), the [[LabAdviser/314/Preparation_314-307/Soft-matter#Leica_EM_FC7_Cryo-Ultramicrotome|Cryo-Ultramicrotome]] can be used for that purpose. | ||
[[File:20210519_Microalgae_K2_007-1.jpg|500px|left|thumb|STEM imaging of a cross section of Microalgae, 100 nm thick section]]<br clear="all" /> | [[File:20210519_Microalgae_K2_007-1.jpg|500px|left|thumb|STEM imaging of a cross section of Microalgae, 100 nm thick section]]<br clear="all" /> | ||
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[[File:Blue cheese_002-1.jpg|500px|left|thumb|Cryo-SEM imaging of Blue cheese]]<br clear="all" /> | [[File:Blue cheese_002-1.jpg|500px|left|thumb|Cryo-SEM imaging of Blue cheese]]<br clear="all" /> | ||
[[File:Cryo-SEM of Mucus in rat intestine-1.jpg|500px|left|thumb|Cryo-SEM imaging of Mucus in rat intestine]]<br clear="all" /> | [[File:Cryo-SEM of Mucus in rat intestine-1.jpg|500px|left|thumb|Cryo-SEM imaging of Mucus in rat intestine]]<br clear="all" /> | ||
[[File: | [[File:20220310_Lotus leaf_023-1.jpg|500px|left|thumb|Cryo-SEM imaging of plant leaf surface]]<br clear="all" /> | ||
== EDS (Energy Dispersive X-ray Spectroscopy) == | == EDS (Energy Dispersive X-ray Spectroscopy) == | ||
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[[File:EDS Bacteria and Au nanoparticle.JPG|1000px|left|thumb|EDS mapping of bacteria and gold nanoparticle]]<br clear="all" /> | [[File:EDS Bacteria and Au nanoparticle.JPG|1000px|left|thumb|EDS mapping of bacteria and gold nanoparticle]]<br clear="all" /> | ||
[[File:Microalgae with iron nanoparticles .jpg|1000px|left|thumb|SEM analysis and EDS mapping of microalgae with iron nanoparticles]]<br clear="all" /> | |||