LabAdviser/314/Microscopy 314-307/Technique/Holo/Off-axis ETEM: Difference between revisions
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# If you see a blister on the biprism, it means the biprism is contaminated at this region. Move the biprism by adjusting the knobs on the SAD aperture until the blister is not there | # If you see a blister on the biprism, it means the biprism is contaminated at this region. Move the biprism by adjusting the knobs on the SAD aperture until the blister is not there | ||
#* You don’t want a curved interference pattern, the lines should be straight | #* You don’t want a curved interference pattern, the lines should be straight | ||
# Do not jump around or cause vibrations | # Do not jump around or cause vibrations → Move the chair and yourself away from the desk if necessary | ||
# If the biprism is not stable, even after waiting, tab either of the knobs of the SAD ever so slightly. It is possible the SAD aperture is moving, tabbing will help it fall into a stable position. | # If the biprism is not stable, even after waiting, tab either of the knobs of the SAD ever so slightly. It is possible the SAD aperture is moving, tabbing will help it fall into a stable position. | ||
# Name the reference and object holograms appropriately. I usually have a string for the file along the lines: Sample_Magnification_BiprismVoltage_ExposureTime_Obj_Number for object holograms, and “Obj” is “Ref” for reference holograms. | # Name the reference and object holograms appropriately. I usually have a string for the file along the lines: Sample_Magnification_BiprismVoltage_ExposureTime_Obj_Number for object holograms, and “Obj” is “Ref” for reference holograms. | ||
#* You need to know the biprism voltage and exposure time for later documentation, since these values determine the width and carrier frequency and are always reported in literature. | #* You need to know the biprism voltage and exposure time for later documentation, since these values determine the width and carrier frequency and are always reported in literature. | ||