LabAdviser/314/Preparation 314-307/Soft-matter/Ultramicrotomy: Difference between revisions

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'''Feedback to this page''': '''[mailto:labadviser@nanolab.dtu.dk?Subject=Feed%20back%20from%20page%20http://labadviser.nanolab.dtu.dk/index.php/LabAdviser/314/Preparation_314-307/Soft-matter/Ultramicrotomy click here]'''
(''content by Marie Karen Tracy Hong Lin @DTU Nanolab, 2020'')
[[Category:314]]
[[Category:314-Preparation]]
=Procedure of ultra-sectionning=
=Procedure of ultra-sectionning=


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[[File:1.jpg|400px|left|thumb|1. Trimming resin block using Trim diamond knife]]<br />
[[File:1.jpg|400px|left|thumb|1. Trimming resin block using Trim diamond knife]]<br />


<br clear="all" />
== Step 2: Mount sample to the ultramicrotome ==
<br clear="all" />
== Step 3: Mount glass or diamound knife to knife holder ==
<br clear="all" />


== Step 2: Ultra-sectioning of sample ==
== Step 4: Ultra-sectioning of sample ==
[[File:8 (2).jpg|400px|left|thumb|2. Ultra-sectionning of sample using diamond knife with water boat]]<br />
[[File:8 (2).jpg|400px|left|thumb|4. Ultra-sectionning of sample using diamond knife with water boat]]<br />


<br clear="all" />


== Step 3: Collecting sections ==
== Step 5: Fishing sections ==
[[File:9 (2).jpg|400px|left|thumb|3. Collecting sections using a Perfect loop]]<br />
[[File:9 (2).jpg|400px|left|thumb|5. Collecting sections using a Perfect loop]]<br />


<br clear="all" />


== Step 4: Transfering sections ==
== Step 6: Transfering sections to EM grid ==
[[File:5 (2).jpg|400px|left|thumb|4. Transfering sections on a grid]]<br />
[[File:5 (2).jpg|400px|left|thumb|6. Transfering sections on a grid]]<br />

Latest revision as of 10:12, 17 July 2023

Feedback to this page: click here (content by Marie Karen Tracy Hong Lin @DTU Nanolab, 2020)

Procedure of ultra-sectionning

Step 1: Trimming resin block

1. Trimming resin block using Trim diamond knife



Step 2: Mount sample to the ultramicrotome


Step 3: Mount glass or diamound knife to knife holder


Step 4: Ultra-sectioning of sample

4. Ultra-sectionning of sample using diamond knife with water boat



Step 5: Fishing sections

5. Collecting sections using a Perfect loop



Step 6: Transfering sections to EM grid

6. Transfering sections on a grid


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