View source for LabAdviser/314/Preparation 314-307

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==Optical Microscopes==
	 

1) '''Olympus BX51 colorview'''
	 
[http://www.physics.umu.se/digitalAssets/140/140383_optical-microscope--olympus-bx51-.pdf  Operation Manual]
	 
[[File:IMG_3671.JPG|200px|left|thumb|Location Building 307, Prep Lab 109]]<br />
	 
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2) '''Olympus SZ61 stereo Zoom Microscope'''
	 
[http://www.olympus-lifescience.com/en/microscopes/stereo/sz61/ Brochure]
	 
[[File:IMG_3673.JPG|left|thumb|Location Building 307, Prep Lab 109]]<br />
	 
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3) '''Leica MZ75 stereo microscope'''
	 
The Leica MZ75 stereo microscope offers 0.63x to 5x zoom and helps in viewing grids or cutting samples before the cryo-ultramicrotome procedures.

Operating manual:
[[:File:Operation Manual Leica_MZ75.pdf]]

For further information about the machine usage contact mktracy@dtu.dk.
	 
[[File:Leica MZ75.jpg|400px|left|thumb|Location at DTU Nanolab Building 307, Room 906]]
	 
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==Ion milling ==
	 
1) Fischione Low Angle Ion Milling and Polishing System Model 1010. The Fischione Ion Mill is used for producing TEM specimens with large electron transparent areas. Samples are pre-thinned by e.g. mechanical grinding and polishing or chemical polishing. The milling or polishing is performed by argon ions. The instrument has two independently adjustable Hollow Anode Discharge (HAD) argon ion sources, which can be operated from 0.5 to 6.0 kV with currents from 3 mA to 8 mA. The milling angle can be adjusted in the range of 0° to 45°. Tuning the milling parameters allows for coarse or gentle milling and the goal is to get clean samples free of physical or chemical artifacts
	 
[[:File:Operation Manual Fischione_1010.pdf]]
	 
[[File:IMG_3674.JPG|300px|left|thumb|Location Building 307, Prep Lab 109]].<br />
	 
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2) Fischione NanoMill model 1040. This TEM specimen preparation system is an excellent tool for creating the high-quality thin specimens needed for advanced transmission electron microscopy imaging and analysis. It is ideal for both post-FIB (focused ion beam) processing and the enhancement of conventionally prepared specimens.
	 
[http://www.fischione.com/products/ion-beam-preparation/model-1040-nanomill%C2%AE-tem-specimen-preparation-system Operation Manual]
	 
[[File:IMG_3679.JPG|250px|left|thumb|Location Building 314, Common Area]]
	 
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==Pumping stations ==
	 
1) Pumping Cube for single TEM specimen holder.
	 
[[File:IMG_3663.JPG|300px|left|thumb|Location Building 314, Prep Room 01]]<br />
	 
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2) Gatan Turbo Pumping Station model 655. Capacity 4 specimen holders.The turbo pumping station is a completely self-contained, compact, benchtop pumping station designed to regenerate the sorb material in cooling and cryo-transfer holder dewars and for vacuum storage of transmission electron microscope (TEM) holders or samples.
	 
[http://www.gatan.com/products/tem-specimen-holders/turbo-pumping-station/ Brochure]
	 
[[File:IMG_3677.JPG|250px|left|thumb|Location Building 314, Prep Room 03]]<br />
	 
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==Polishing==
	 
1) Struers Labopol -25.
	 
[http://www.struers.com/default.asp?top_id=2&main_id=93&sub_id=145&doc_id=534  Brochure]
	 
[[File:labopol25.png|200px|left|thumb|Location Building 307, Prep Lab 109]]<br />
	 
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2) TechPrep Allied.The MultiPrep™ System enables precise semiautomatic sample preparation of a wide range of materials for microscopic (optical, SEM, FIB, TEM, AFM, etc.) evaluation. 

Capabilities include parallel polishing, angle polishing, site-specific polishing or any combination thereof. It provides reproducible results by eliminating inconsistencies between users, regardless of their skill. 
	 
[http://www.alliedhightech.com/Equipment/multiprep-polishing-system-8/ Brochure]
	 
[[File:TechPrep.png|200px]|left|thumb|Location Building 307, Prep Lab 109]]<br />
	 
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3) Struer Labopol -5.
	 
[[File:Labopol5.png|200px]|left|thumb|Location Building 307, Prep Lab 109]]<br />
	 
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4) Struer Labopol -4.
	 
[[File:Labopol4.png|200px|left|thumb|Location Building 307, Prep Lab 109]]<br />
	 
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==Saws==
	 
1) Struers Minitom. 
	 
[http://www.struers.com/default.asp?doc_id=262  Brochure]
	 
[[File:IMG_3664.JPG|200px|left|thumb|Location Building 307, Prep Lab 109]]<br />
	 
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2) Well Precision Diamond Wire Saw.
	 
[http://www.hititmetal.com/urun/metalografi/3000_seri.pdf  Brochure]
	 
[[File:IMG_3665.JPG|200px|left|thumb|Location Building 307, Prep Lab 109]]<br />
	 
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==Plasma cleaner & Pumping Station==
	 
1) Fischione Plasma Cleaner model 1020. The Plasma Cleaner automatically and quickly removes organic contamination (hydrocarbon) from electron microscopy specimens and specimen holders. A low energy, reactive gas plasma cleans without changing the specimen’s elemental composition or structural characteristics. The Plasma Cleaner features easy-to-use front panel controls and an oil-free vacuum system for optimal processing.
	 
[http://www.fischione.com/products/contamination-solutions/model-1020-plasma-cleaner Brochure]
	 
[[File:IMG_3682.JPG|250px|left|thumb|Location Building 307, Prep Room 03]].<br />
	 
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==Coaters & Accessories==
	 
1) Cressington 208 Carbon Coater. 
	 
[http://cressington.com/product_208c.html  Brochure]
	 
[[File:IMG_3684.JPG|250px|left|thumb|Location Building 314, Prep Room 03]]<br />
	 
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2) Quorum Q150T Sputter Coater and Glow discharge

[http://labmanager.dtu.dk/view_binary.php?fileId=3020 Manual]

[[File:xxx.jpg|250px|left|thumb|Location Building 314, Prep Room 03]]<br />

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<!-- 2) Cressington 208HR Sputter Coater.
	 
[http://cressington.com/spec_208hr.html  Brochure]
	 
[[File:IMG_3685.JPG|250px|left|thumb|Location Building 314, Prep Room 01]]<br />
	 
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Accessory for 208HR Sputter Coater:
	 
Glow Discharge Head 
	 
[[File:IMG_3687.JPG|250px|left|thumb|Location Building 314, Prep Room 01]]<br />
	 
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==Sonicators ==
	 
1) Ultrasonic Branson 2510. 
	 
[https://store-19uyxzz.mybigcommerce.com/content/Branson/B_SeriesManual.pdf  Brochure]
	 
[[File:IMG_3676.JPG|250px|left|thumb|Location Building 307, Prep Prep Lab 109]]<br />
	 
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2) VWR Ultrasonic Cleaner.
	 
[http://www.massetrecovery.com/pictures12/VWR%20Ultrasonic%20cleaner.pdf  Brochure]
	 
[[File:IMG_3678.JPG|250px|left|thumb|Location Building 314, Common Area]]<br />
	 
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3) Sonic Dismembrator.

For further information on the machine usage contact: mktracy@dtu.dk.

[[File:xxx.jpg|250px|left|thumb|Location Building 314, Room 040]]<br />

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==Hot plates ==
	 
Nuova hot plate. 
	 
[http://www.laboratory-equipment.com/laboratory-equipment/nuova-hot-plates-stirrers.php  Brochure]
	 
[[File:IMG_3672.JPG|250px|left|thumb|Location Building 307, Prep Lab 109]]<br />
	 
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==Plunger==
<!--The FEI Vitrobot™ is used for vitrifying (freezing) single particle samples for cryo-TEM. The Vitrobot™ is fully automated and performs the cryo-fixation process at constant conditions (temperature, relative humidity, blotting and freezing velocity), which ensures high quality cryo-fixation. The sample is pipetted on a TEM grid, blotted and then plunged in liquid ethane, which is cooled with liquid nitrogen. The FEI Vitrobot™ Data Sheet is here [[:File:Vitrobot_Mark_IV_Datasheet.pdf]] and an example of the APV can be found here [[:File:APV_Vitrobot.pdf]].
The Vitrobot™ is located in building 314, in the Soft Mater (toxic) Lab. For further information on the machine usage contact ramona.mateiu@cen.dtu.dk.
[[File:Vitrobot.jpg|200px|thumb|left|DTU Cens FEI Vitrobot]]<br /> -->

'''Leica EM GP2'''

The Leica plunge freezer (EM GP2) is used to vitrifying (freezing) samples for cryo-TEM under liquid ethane temperature.


Operating manual:[[:File:Leica EM GP2 Operating manual.pdf]]


For further information on the machine usage contact merafik@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=135492&cpid=245590&tab=0] or mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].
 

[[File:Plunge Freezer.jpg|400px|left|thumb|Location at DTU Nanolab Building 314, Room 014 (Tecnai T20)]]<br /> 
 

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== Microtomes ==
1) Ultramicrotome

'''RMC MT-7 Microtome'''

Our RMC MT-7 Microtome is a vintage piece of equipment and can be used for planning specimens for SEM/EDX and/or for cutting relative thin slices (approx. 200nm thick) for TEM. First, the specimen needs to be embedded into a resin or an epoxy, and then can be sliced with a glass or diamond knife.  At DTU Nanolab Cen we can offer only glass knifes. The Risk Assessment for the RMC MT-7 Microtome can be found here [[:File:APV RMC Microtome.pdf]]
The RMC MT-7 Microtome is located in building 314, in the Soft Mater preparation lab. 

For further information about the machine usage contact afull@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=65646&cpid=173468&tab=2&qt=dtupublicationquery].

[[File: RMC Microtome.jpg|200px|thumb|left|DTU Nanolab RMC MT-7 Microtome]]

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'''Leica EM UC7'''

The Leica ultramicrotome is used for ultra thin sectioning of sample embedded in resin block. Glass or diamond knife is used for ultra sectioning of the samples. 


Operating manual: [[:File:EM UC7 Operating manual.pdf]] 

Risk Assessment: [[:File:Risk assessment-Ultramicrotome EM UC7.docx]]


For further information about the machine usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].

[[File:Leica EM UC7.jpg|400px|left|thumb|Location at DTU Nanolab Building 307, Room 906]]<br />

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2) Cryo-Ultramicrotome

'''Leica EM FC7'''

Operating manual: [[:File:EM FC7 Operating manual.pdf]] 

Risk Assessment: [[:File:Risk assessment-Cryo-Ultramicrotome EM FC7.docx]]


For further information about the machine usage contact merafik@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=135492&cpid=245590&tab=0] or mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0]. 

[[File:Leica EM FC7.jpg|400px|left|thumb|Location at DTU Nanolab Building 307, Room 906]]<br />

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==Critical point dryer ==

'''Leica EM CPD300'''

The Critical Point Drier (CPD) is used for drying samples for the SEM. The machine uses the fact that at the critical point the solvent can be converted from liquid to gas without crossing the interfaces between liquid and gas (no surface tension) and hence no sample drying artefacts. CO2 is the solvent of choice as its triple point is suitable for biological samples (31°C and 74 bars). The CO2 is not miscible with water; therefore the sample should be dissolved in acetone or ethanol.


Operating manual: [[:File:EM CPD300 Operating manual.pdf]] 

Risk Assessment: [[:File:Risk assessment-EM CPD300.docx]].


For further information on the machine usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].

[[File: Leica EM CPD300.jpg|200px|thumb|left|Location at DTU Nanolab Building 314]]

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==Centrifuge==
The Mini Spinner Eppendorf is a desktop centrifuge with supplied rotor with maximum speed/force 13,400rpm/12,100xG. It has a 30-minute timer, which is settable in 15-sec increments. It has a short-spin mode fixed at maximum speed. The Mini Spinner Eppendorf is located in building 314, in the Soft Mater (toxic) Lab. 

For further information on the machine usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].

[[File: MINI Spin.jpg|200px|thumb|left|Location at DTU Nanolab Building 314]]

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==pH meter ==
If you are planning on doing chemical fixation on biological samples you will probably need to make buffers and use a pH-meter. The pH-meter (model pH 1000L) is located in building 314, in the Soft Mater (toxic) Lab and it is dedicated to “Soft Matter” related work. 

For further information on the machine usage contact mktracy@dtu.dk [https://www.dtu.dk/english/service/phonebook/person?id=141983&cpid=260636&tab=0].

[[File: pH meter.jpg|200px|thumb|left|Location at DTU Nanolab Building 314]]

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