Specific Process Knowledge/Characterization/MicroSpectroPhotometer (Craic 20/30 PV): Difference between revisions
| Line 69: | Line 69: | ||
To create an absorption spectrum from two single-beam spectra, you can use the following formula: | To create an absorption spectrum from two single-beam spectra, you can use the following formula: | ||
<math>A=-\log\frac{S-D}{R-D}</math> | |||
In this formula, R represents the single-beam spectrum of the reference material, S represents the single-beam spectrum of the sample, and D represents the dark counts of the system. | In this formula, R represents the single-beam spectrum of the reference material, S represents the single-beam spectrum of the sample, and D represents the dark counts of the system. | ||
| Line 76: | Line 76: | ||
Alternatively, you can generate an absorption spectrum from transmission data using the following formula: | Alternatively, you can generate an absorption spectrum from transmission data using the following formula: | ||
<math>A=2-\log(T(\%))</math> | |||
This formula allows you to convert percent transmittance (%T) values into absorbance. It is particularly useful for processing data that has already been collected | This formula allows you to convert percent transmittance (%T) values into absorbance. It is particularly useful for processing data that has already been collected | ||