LabAdviser/314/Microscopy 314-307/TEM/Tips/TVIPS camera - Revision history

Jenk at 09:48, 8 September 2025

2025-09-08T09:48:22Z

← Older revision		Revision as of 11:48, 8 September 2025
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	(''content by Jens Kling, September 2021'')		(''content by Jens Kling, September 2021'')
	~~[[Category:314]]~~
	~~[[Category:314-Microscopy]]~~

	~~<span style="background:#FF2800">THIS PAGE IS UNDER CONSTRUCTION</span>[[image:Under_construction.png\|200px]]~~

			[[index.php?title=Category:314]]
			[[index.php?title=Category:314-Microscopy]]

	'''TVIPS camera and EMplify software'''		'''TVIPS camera and EMplify software'''

Jenk at 14:12, 7 March 2022

2022-03-07T14:12:54Z

← Older revision		Revision as of 16:12, 7 March 2022
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	== Tips and bugs in EMplified ==		== Tips and bugs in EMplified ==
	''Status: October 2021''		''Status: October 2021''


			=== Don't burn scalebar ===
			Don't burn a scalebar into your 16bit image! This will result in the following issue:
			[[File:Example_burned-scalebar_TVIPS.png\|250px\|thumb\|left\|Burned scalebar in 16bit image]]<br clear="all" />
			There is no need to burn the scalebar in the image. The 16bit tiff-file contains the image calibration to add a scalebar in ImageJ.
			<br />
			<br />

Jenk: /* The software or camera doesn't respond properly or freezes */

2021-11-03T14:15:10Z

The software or camera doesn't respond properly or freezes

← Older revision		Revision as of 16:15, 3 November 2021
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	** Wait 10 sek and push it again (should become green again)		** Wait 10 sek and push it again (should become green again)
	** Restart the EMplified software		** Restart the EMplified software
	When EMplified crashed~~, the unsaved images are temporarily saved under '''C:\temp\EMplified\Unsorted''' (This folder will be deleted at the next re-start of the software).<br />~~		When EMplified crashed and the software is re-started after a crash, EMplified will also ask if unsaved images should be recovered.<br />
	~~When~~ the software is re-started after a crash, EMplified will also ask if unsaved images should be recovered.<br />
	If this procedure did not succeed, repeat the re-start but also re-start the TVIPS PC.		If this procedure did not succeed, repeat the re-start but also re-start the TVIPS PC.
	<br />		<br />

Jenk: /* Tips and bugs in EMplified */

2021-11-03T13:35:34Z

Tips and bugs in EMplified

← Older revision		Revision as of 15:35, 3 November 2021
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	<br />		<br />

	=== The acquired image becomes white ===		=== The acquired image becomes white or black===
	This is a bug in the current EMplified version. It can happen that the images in the Viewport become completely white or black.		This is a bug in the current EMplified version. It can happen that the images in the Viewport become completely white or black.
	This has to do with the contrast/brightness setting of the '''Histogram'''. In the "Standard new" layout, you can find the global Histogram on the left hand side. The Histogram is active on different windows depending on the operation:		This has to do with the contrast/brightness setting of the '''Histogram'''. In the "Standard new" layout, you can find the global Histogram on the left hand side. The Histogram is active on different windows depending on the operation:
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	* When the Live View is '''NOT''' running, it is active on the "Viewport".		* When the Live View is '''NOT''' running, it is active on the "Viewport".
	When the bug happens and you want to see your acquired image, stop the Live View and click on '''Reset C/B''' in the Histogram control panel. Now, the image in the Viewport should look normal. When the Live View is restarted, the image might turn white or black again. As long as you save in 16bit format, the saved images are fine. If you save in 8bit format, there might be an issue with the saved files (they are white or black as well).<br /><br />		When the bug happens and you want to see your acquired image, stop the Live View and click on '''Reset C/B''' in the Histogram control panel. Now, the image in the Viewport should look normal. When the Live View is restarted, the image might turn white or black again. As long as you save in 16bit format, the saved images are fine. If you save in 8bit format, there might be an issue with the saved files (they are white or black as well).<br /><br />
	So, the strong suggestion is: '''Save all images in 16bit format!'''<br ~~clear="all"~~ />		So, the strong suggestion is: '''Save all images in 16bit format!'''
			<br />
	<br />		<br />

	=== I can't see anything in the ~~Powerspectrum~~ ===		=== I can't see anything in the Power Spectrum ===
			This seems to be a bug in the current version as well. It has to do with the zoom factor in the Power Spectrum.<br />
			Using the mousewheel, you can zoom in and out in the Power Spectrum at the mouse pointer position (like in the other windows as well). In the Power Spectrum tool bar, there is an icon for reset the zoom (square icon with two boxes). Click on the icon and the Power Spectrum zoom will reset. Now you should be able to see the whole Power Spectrum again.
			[[File:Power-Spectrum.png\|250px\|thumb\|left\|Power Spectrum]]<br clear="all" />
	<br />		<br />

			=== The software or camera doesn't respond properly or freezes ===
			The software is officially still in "beta"-phase. This means, there are still some bugs and it might shut down unexpected. Please report back any bugs you find.<br />
			In case the software crashes, just follow the steps:
			[[File:Control-box.png\|250px\|thumb\|right\|Power Spectrum]]
			* Close the software (if needed)
			* If a re-start of the software doesn’t help, re-start the camera controller as well:
			** Go to the back of the screens, where the control box for the camera is
			** Push on the Camera button once (switched from green to red)
			** DON’T switch off the cooling!
			** Wait 10 sek and push it again (should become green again)
			** Restart the EMplified software
			When EMplified crashed, the unsaved images are temporarily saved under '''C:\temp\EMplified\Unsorted''' (This folder will be deleted at the next re-start of the software).<br />
			When the software is re-started after a crash, EMplified will also ask if unsaved images should be recovered.<br />
			If this procedure did not succeed, repeat the re-start but also re-start the TVIPS PC.
	<br />		<br />

Jenk: /* Tips and bugs in EMplified */

2021-11-03T12:54:14Z

Tips and bugs in EMplified

← Older revision		Revision as of 14:54, 3 November 2021
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	=== The live view images jumps ===		=== The live view images jumps ===
	This only seems to happen when the ''Drift correction'' in the Live View is activated. Deactivating the feature should resolve the behavior.<br />		This only seems to happen when the '''Drift correction''' in the Live View is activated. Deactivating the feature should resolve the behavior.<br />
	[[File:LiveView_drift-correction.png\|250px\|thumb\|left\|Live View and Drift correction.]]<br clear="all" />		[[File:LiveView_drift-correction.png\|250px\|thumb\|left\|Live View and Drift correction.]]<br clear="all" />
	<br />		<br />

	=== The acquired image becomes white ===		=== The acquired image becomes white ===
	~~Dear users, There~~ is a bug in EMplified ~~(TVIPS software), which a couple of you experienced already:~~ It can happen that the images in the Viewport become completely white or black. This has to do with the contrast/brightness setting of the Histogram. In the "Standard new" layout, you can find the global Histogram on the left hand side. The Histogram is active on different windows depending on the operation: - When the Live ~~view~~ is running, it is active on the "Live View" window or the "Power Spectrum", depending on which window is selected. - When the Live ~~view~~ is NOT running, it is active on the "~~ViewPort~~". When the bug happens and you want to see your acquired image, stop the Live ~~view~~ and click on "Reset C/B" in the Histogram control panel. Now, the image in the ~~ViewPort~~ should look normal. When the ~~live view~~ is restarted, the image might turn white or black again. As long as you save in 16bit format, the saved images are fine. If you save in 8bit format, there might be an issue with the saved files (they are white or black as well). So, the strong suggestion is: Save all images in 16bit format! ~~TVIPS knows about the issue and is working on it.~~		This is a bug in the current EMplified version. It can happen that the images in the Viewport become completely white or black.
	<br />		This has to do with the contrast/brightness setting of the '''Histogram'''. In the "Standard new" layout, you can find the global Histogram on the left hand side. The Histogram is active on different windows depending on the operation:
			* When the '''Live View''' is running, it is active on the "Live View" window or the "Power Spectrum", depending on which window is selected.
			* When the Live View is '''NOT''' running, it is active on the "Viewport".
			When the bug happens and you want to see your acquired image, stop the Live View and click on '''Reset C/B''' in the Histogram control panel. Now, the image in the Viewport should look normal. When the Live View is restarted, the image might turn white or black again. As long as you save in 16bit format, the saved images are fine. If you save in 8bit format, there might be an issue with the saved files (they are white or black as well).<br /><br />
			So, the strong suggestion is: '''Save all images in 16bit format!'''<br clear="all" />
	<br />		<br />

	=== I can't see anything in the Powerspectrum ===		=== I can't see anything in the Powerspectrum ===
	<br />		<br />
	<br />		<br />

Jenk: /* Tips and bugs in EMplified */

2021-11-03T12:48:23Z

Tips and bugs in EMplified

← Older revision		Revision as of 14:48, 3 November 2021
Line 25:		Line 25:


	=== The live view images jumps === <br />		=== The live view images jumps ===
			This only seems to happen when the ''Drift correction'' in the Live View is activated. Deactivating the feature should resolve the behavior.<br />
			[[File:LiveView_drift-correction.png\|250px\|thumb\|left\|Live View and Drift correction.]]<br clear="all" />
	<br />		<br />
	=== The acquired image becomes white === <br />
			=== The acquired image becomes white ===
			Dear users, There is a bug in EMplified (TVIPS software), which a couple of you experienced already: It can happen that the images in the Viewport become completely white or black. This has to do with the contrast/brightness setting of the Histogram. In the "Standard new" layout, you can find the global Histogram on the left hand side. The Histogram is active on different windows depending on the operation: - When the Live view is running, it is active on the "Live View" window or the "Power Spectrum", depending on which window is selected. - When the Live view is NOT running, it is active on the "ViewPort". When the bug happens and you want to see your acquired image, stop the Live view and click on "Reset C/B" in the Histogram control panel. Now, the image in the ViewPort should look normal. When the live view is restarted, the image might turn white or black again. As long as you save in 16bit format, the saved images are fine. If you save in 8bit format, there might be an issue with the saved files (they are white or black as well). So, the strong suggestion is: Save all images in 16bit format! TVIPS knows about the issue and is working on it.
			<br />
			<br />
			=== I can't see anything in the Powerspectrum ===
	<br />		<br />
	~~=== I can't see anything in the Powerspectrum === <br />~~
	<br />		<br />

Jenk: /* Tips and bugs in EMplified */

2021-11-03T12:05:50Z

Tips and bugs in EMplified

← Older revision		Revision as of 14:05, 3 November 2021
Line 25:		Line 25:


	* The live view images jumps <br />		=== The live view images jumps === <br />
	<br />		<br />
	* The acquired image becomes white <br />		=== The acquired image becomes white === <br />
	<br />		<br />
	* I can't see anything in the Powerspectrum <br />		=== I can't see anything in the Powerspectrum === <br />
	<br />		<br />

Jenk: /* XF416 camera */

2021-10-08T07:27:46Z

XF416 camera

← Older revision		Revision as of 09:27, 8 October 2021
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	* CMOS camera 4096x4096px resolution with minimum exposure time of 41ms (24fps).<br />		* CMOS camera 4096x4096px resolution with minimum exposure time of 41ms (24fps).<br />
	* 15.5x15.5µm<sup>2</sup> pixel size<br />		* 15.5x15.5µm<sup>2</sup> pixel size<br />
			<br />

	== additional software features in EMplified ==		== additional software features in EMplified ==

Jenk: /* XF416 camera */

2021-10-08T07:27:31Z

XF416 camera

← Older revision		Revision as of 09:27, 8 October 2021
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	* CMOS camera 4096x4096px resolution with minimum exposure time of 41ms (24fps).<br />		* CMOS camera 4096x4096px resolution with minimum exposure time of 41ms (24fps).<br />
	* 15.5x15.5µm~~&sup2~~ pixel size<br />		* 15.5x15.5µm<sup>2</sup> pixel size<br />


	== additional software features in EMplified ==		== additional software features in EMplified ==

Jenk at 06:31, 8 October 2021

2021-10-08T06:31:13Z

← Older revision		Revision as of 08:31, 8 October 2021
Line 15:		Line 15:
	== XF416 camera ==		== XF416 camera ==

			* CMOS camera 4096x4096px resolution with minimum exposure time of 41ms (24fps).<br />
			* 15.5x15.5µm&sup2 pixel size<br />


			== additional software features in EMplified ==

	== Tips and bugs in EMplified ==		== Tips and bugs in EMplified ==