LabAdviser/314/Microscopy 314-307/TEM/ETEM/OneView - Revision history

Jenk at 07:30, 23 August 2023

2023-08-23T07:30:43Z

← Older revision		Revision as of 09:30, 23 August 2023
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	'''Feedback to this page''': '''[mailto:labadviser@nanolab.dtu.dk?Subject=Feed%20back%20from%20page%20http://labadviser.nanolab.dtu.dk/index.php/LabAdviser/314/ETEM/OneView click here]'''		'''Feedback to this page''': '''[mailto:labadviser@nanolab.dtu.dk?Subject=Feed%20back%20from%20page%20http://labadviser.nanolab.dtu.dk/index.php/LabAdviser/314/ETEM/OneView click here]'''

	(''content by Jens Kling, September 2021'')		(''content by Jens Kling @DTU Nanolab, September 2021'')
	[[Category:314]]		[[Category:314]]
	[[Category:314-Microscopy]]		[[Category:314-Microscopy]]

Jenk: /* Operation */

2022-09-20T08:26:55Z

Operation

← Older revision		Revision as of 10:26, 20 September 2022
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	[[image:Gatan_OneView_1.png\|400x400px\|right\|thumb\|GMS3 main window.]]		[[image:Gatan_OneView_1.png\|400x400px\|right\|thumb\|GMS3 main window.]]

	1. If the computer for the OneView camera is not running (large screen), start the computer. It is located in the technical room behind the microscope (large computer with a lot of hard drive slots). The login ~~is:<br />~~		1. If the computer for the OneView camera is not running (large screen), start the computer. It is located in the technical room behind the microscope (large computer with a lot of hard drive slots). The login can be found on the whiteboard.
	~~: user: '''Administrator'''<br />~~
	~~: PW: '''$admin'''~~
	<br />		<br />
	2. If GMS3 (Gatan Microscopy Suite; also known as Digital Micrograph) is not running yet, click on the GMS icon and acknowledge any information message (elevated privileges; using potentially harmful plug-ins).<br />		2. If GMS3 (Gatan Microscopy Suite; also known as Digital Micrograph) is not running yet, click on the GMS icon and acknowledge any information message (elevated privileges; using potentially harmful plug-ins).<br />
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	* Each frame will be saved hierarchically in the folder under '''Hour_xx''', '''Minute_xx''', '''Second_xx'''.<br />		* Each frame will be saved hierarchically in the folder under '''Hour_xx''', '''Minute_xx''', '''Second_xx'''.<br />
	<br />		<br />


	== Additional information ==		== Additional information ==

Jenk at 11:57, 4 July 2022

2022-07-04T11:57:13Z

@@ Line 22: / Line 22: @@
 - In our setup, the OneView camera exclusively uses the pre-specimen shutter in idle state (beam on the camera but camera not running), which means the sample is not illuminated.<br />
 <br />
 == Operation ==

Jenk: /* Additional information */

2022-03-07T10:47:26Z

Additional information

← Older revision		Revision as of 12:47, 7 March 2022
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	*[[LabAdviser/314/Microscopy 314-307/TEM/ETEM/reference-images\|Dark and gain reference on OneView]]		*[[LabAdviser/314/Microscopy 314-307/TEM/ETEM/reference-images\|Dark and gain reference on OneView]]

			*[[LabAdviser/314/Microscopy 314-307/TEM/ETEM/OneView restart\|Communication/Connection problems]]

Jenk: /* Additional information */

2022-02-22T09:00:17Z

Additional information

← Older revision		Revision as of 11:00, 22 February 2022
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	*[[LabAdviser/314/Microscopy 314-307/TEM/ETEM/dose-calibration\|Set dose calibration for OneView]]		*[[LabAdviser/314/Microscopy 314-307/TEM/ETEM/dose-calibration\|Set dose calibration for OneView]]

			*[[LabAdviser/314/Microscopy 314-307/TEM/ETEM/reference-images\|Dark and gain reference on OneView]]


	<!-- Go to "Customs" and "SetDoseCalibrationTag_OneView_mod-DTU" <br />		<!-- Go to "Customs" and "SetDoseCalibrationTag_OneView_mod-DTU" <br />
	[[File:Oneview_1.png\|600px\|center\|thumb\|Run script]]		[[File:Oneview_1.png\|600px\|center\|thumb\|Run script]]
	<br clear="all" /> -->		<br clear="all" /> -->

Jenk at 08:58, 22 February 2022

2022-02-22T08:58:58Z

← Older revision		Revision as of 10:58, 22 February 2022
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	[[image:Gatan_OneView_1.png\|400x400px\|right\|thumb\|GMS3 main window.]]		[[image:Gatan_OneView_1.png\|400x400px\|right\|thumb\|GMS3 main window.]]

	1. If ~~GMS3 (Gatan Microscopy Suite; also known as Digital Micrograph)~~ is not running ~~on the separate computer~~ (large screen) ~~yet~~, ~~click on~~ the ~~GMS icon and acknowledge any information message~~.<br />		1. If the computer for the OneView camera is not running (large screen), start the computer. It is located in the technical room behind the microscope (large computer with a lot of hard drive slots). The login is:<br />
			: user: '''Administrator'''<br />
			: PW: '''$admin'''
	<br />		<br />
	2. ~~Check in~~ the ~~'''Camera Monitor''' that the temperature is~~ -~~5°C. Else, wait until it reaches this temperature~~.<br />		2. If GMS3 (Gatan Microscopy Suite; also known as Digital Micrograph) is not running yet, click on the GMS icon and acknowledge any information message (elevated privileges; using potentially harmful plug-ins).<br />
	<br />		<br />
	3. ~~On the Microscope PC,~~ the ~~software~~ '''~~Gatan Remote~~''' ~~should be running in~~ the ~~background~~. ~~If not~~, ~~start~~ this ~~software~~.<br />		3. Check in the '''Camera Monitor''' that the temperature is -5°C. Else, wait until it reaches this temperature.<br />
	<br />		<br />
	4. ~~Make sure you are an ''~~'~~Power User~~''~~'. Click on '''Help~~''' in the ~~top task bar and select '''Power User''' under '''User Mode'''~~. ~~Now you have all relevant settings and properties available~~.<br />		4. On the Microscope PC, the software '''Gatan Remote''' should be running in the background. If not, start this software.<br />
	<br />		<br />
	5. On the left hand side under '''Microscope''', GMS shows the visualization of the TEM. Here, HT and Magnification should be the same as in the Microscope PC. Clicking on '''Valves''' will open or close the Column Valves, '''Blank''' will blank the beam before the sample, clicking on the '''Phosphorous screen''' will lift or lower the screen. Right click on the '''OneView''' camera icon opens a context menu, where the camera can be inserted or retracted.<br /><br />		5. Make sure you are an '''Power User'''. Click on '''Help''' in the top task bar and select '''Power User''' under '''User Mode'''. Now you have all relevant settings and properties available.<br />
			<br />
			6. On the left hand side under '''Microscope''', GMS shows the visualization of the TEM. Here, HT and Magnification should be the same as in the Microscope PC. Clicking on '''Valves''' will open or close the Column Valves, '''Blank''' will blank the beam before the sample, clicking on the '''Phosphorous screen''' will lift or lower the screen. Right click on the '''OneView''' camera icon opens a context menu, where the camera can be inserted or retracted.<br /><br />
	!!! The Ultrascan camera needs to be retracted before inserting the OneView camera !!!<br />		!!! The Ultrascan camera needs to be retracted before inserting the OneView camera !!!<br />
	<br />		<br />
	6. On the frontpage right hand side, you find the '''Technique Manager'''. Under '''TEM''' you find '''TEM imaging''' and '''In-situ imaging'''.<br />		7. On the frontpage right hand side, you find the '''Technique Manager'''. Under '''TEM''' you find '''TEM imaging''' and '''In-situ imaging'''.<br />
	<br />		<br />

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	* Each frame will be saved hierarchically in the folder under '''Hour_xx''', '''Minute_xx''', '''Second_xx'''.<br />		* Each frame will be saved hierarchically in the folder under '''Hour_xx''', '''Minute_xx''', '''Second_xx'''.<br />
	<br />		<br />


	== Additional information ==		== Additional information ==

Jenk: /* Imaging mode */

2021-10-28T13:10:41Z

Imaging mode

← Older revision		Revision as of 15:10, 28 October 2021
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	* This means the signal-to-noise in each individual frame should be high enough to get a decent summed final image.<br />		* This means the signal-to-noise in each individual frame should be high enough to get a decent summed final image.<br />
	<br />		<br />
	* There is an additional feature which can help with low-dose image capture: When changing "Exposure" next to '''Capture''' into "Low dose", it is possible to set a dose rate. Then the camera will expose the camera chip until this dose rate on the image is achieved. In that case, the "real" exposure time is changed, which can help with low intensity on the camera. You should make sure that the dose calibration is set properly.<br />		* There is an additional feature which can help with low-dose image capture: When changing "Exposure" next to '''Capture''' into "Low dose", it is possible to set a dose rate. Then the camera will expose the camera chip until this dose rate on the image is achieved. In that case, the "real" exposure time is changed, which can help with low intensity on the camera. You should make sure that the dose calibration is set properly ([[LabAdviser/314/Microscopy 314-307/TEM/ETEM/dose-calibration\|Set dose calibration for OneView]]).<br />
	<br />		<br />

Jenk: /* Imaging mode */

2021-10-28T13:10:06Z

Imaging mode

← Older revision		Revision as of 15:10, 28 October 2021
Line 58:		Line 58:
	* This means the signal-to-noise in each individual frame should be high enough to get a decent summed final image.<br />		* This means the signal-to-noise in each individual frame should be high enough to get a decent summed final image.<br />
	<br />		<br />
	* There is an additional feature which can help with low-dose image capture: When changing "Exposure" next to '''Capture''' into "Low dose", it is possible to set a dose rate. Then the camera will expose the camera chip until this dose rate on the image is achieved. In that case, the "real" exposure time is changed, which can help with low intensity on the camera.<br />		* There is an additional feature which can help with low-dose image capture: When changing "Exposure" next to '''Capture''' into "Low dose", it is possible to set a dose rate. Then the camera will expose the camera chip until this dose rate on the image is achieved. In that case, the "real" exposure time is changed, which can help with low intensity on the camera. You should make sure that the dose calibration is set properly.<br />
	<br />		<br />

Jenk: /* Imaging mode */

2021-10-28T13:09:19Z

Imaging mode

← Older revision		Revision as of 15:09, 28 October 2021
Line 58:		Line 58:
	* This means the signal-to-noise in each individual frame should be high enough to get a decent summed final image.<br />		* This means the signal-to-noise in each individual frame should be high enough to get a decent summed final image.<br />
	<br />		<br />
	* There is an additional feature which can help with low-dose image capture: When changing "Exposure" next to Capture into "Low dose", it is possible to set a dose rate. Then the camera will expose the camera chip until this dose rate on the image is achieved. In that case, the "real" exposure time is changed, which can help with low intensity on the camera.<br />		* There is an additional feature which can help with low-dose image capture: When changing "Exposure" next to '''Capture''' into "Low dose", it is possible to set a dose rate. Then the camera will expose the camera chip until this dose rate on the image is achieved. In that case, the "real" exposure time is changed, which can help with low intensity on the camera.<br />
	<br />		<br />

Jenk: /* Imaging mode */

2021-10-28T13:08:46Z

Imaging mode

← Older revision		Revision as of 15:08, 28 October 2021
Line 58:		Line 58:
	* This means the signal-to-noise in each individual frame should be high enough to get a decent summed final image.<br />		* This means the signal-to-noise in each individual frame should be high enough to get a decent summed final image.<br />
	<br />		<br />
	* There is an additional feature ~~with~~ can help with low-dose image capture: When changing "Exposure" next to Capture into "Low dose", it is possible to set a dose rate. Then the camera will expose the camera chip until this dose rate on the image is achieved. In that case, the "real" exposure time is changed, which can help with low intensity on the camera.<br />		* There is an additional feature which can help with low-dose image capture: When changing "Exposure" next to Capture into "Low dose", it is possible to set a dose rate. Then the camera will expose the camera chip until this dose rate on the image is achieved. In that case, the "real" exposure time is changed, which can help with low intensity on the camera.<br />
	<br />		<br />

	==== Diffraction mode ====		==== Diffraction mode ====