LabAdviser/314/Microscopy 314-307/SEM/QFEG - Revision history

Mktracy: /* Cryo-SEM */

2024-12-05T08:59:24Z

Cryo-SEM

← Older revision		Revision as of 10:59, 5 December 2024
Line 129:		Line 129:
	[[File:Blue cheese_002-1.jpg\|500px\|left\|thumb\|Cryo-SEM imaging of Blue cheese]]<br clear="all" />		[[File:Blue cheese_002-1.jpg\|500px\|left\|thumb\|Cryo-SEM imaging of Blue cheese]]<br clear="all" />
	[[File:Cryo-SEM of Mucus in rat intestine-1.jpg\|500px\|left\|thumb\|Cryo-SEM imaging of Mucus in rat intestine]]<br clear="all" />		[[File:Cryo-SEM of Mucus in rat intestine-1.jpg\|500px\|left\|thumb\|Cryo-SEM imaging of Mucus in rat intestine]]<br clear="all" />
	[[File:~~20211105_Moulded Jam_020~~-1.jpg\|500px\|left\|thumb\|Cryo-SEM imaging of ~~Moulded and expired jam~~]]<br clear="all" />		[[File:20220310_Lotus leaf_023-1.jpg\|500px\|left\|thumb\|Cryo-SEM imaging of plant leaf surface]]<br clear="all" />


	== EDS (Energy Dispersive X-ray Spectroscopy) ==		== EDS (Energy Dispersive X-ray Spectroscopy) ==

Mktracy at 08:55, 5 December 2024

2024-12-05T08:55:09Z

← Older revision		Revision as of 10:55, 5 December 2024
Line 7:		Line 7:
	=QFEG 200 Cryo ESEM (FEI Quanta FEG 200)=		=QFEG 200 Cryo ESEM (FEI Quanta FEG 200)=

	[[~~Image~~:~~FEG~~.jpg\|~~300x300px~~\|right\|thumb\| DTU Nanolab QFEG located in building 307 room 907]]		[[File:202231020_IMG_4763.jpg\|400px\|right\|thumb\| DTU Nanolab QFEG located in building 307 room 907]]

	The QFEG 200 Cryo is an FEI Quanta FEG (Field Emission Gun) scanning electron microscope with a spatial resolution of 2 nm for the ETD detector in high vacuum at 30 keV. The microscope can operate in several modes, such as high vacuum, low vacuum, environmental mode, room temperature, low temperature and cryo. Our QFEG is fitted with an EDS detector, which allows for analytical measurements in all the operation modes. With the cryo transfer system available, it is especially suited for cryo experiments.		The QFEG 200 Cryo is an FEI Quanta FEG (Field Emission Gun) scanning electron microscope with a spatial resolution of 2 nm for the ETD detector in high vacuum at 30 keV. The microscope can operate in several modes, such as high vacuum, low vacuum, environmental mode, room temperature, low temperature and cryo. Our QFEG is fitted with an EDS detector, which allows for analytical measurements in all the operation modes. With the cryo transfer system available, it is especially suited for cryo experiments.

Mktracy at 08:52, 5 December 2024

2024-12-05T08:52:05Z

← Older revision		Revision as of 10:52, 5 December 2024
Line 7:		Line 7:
	=QFEG 200 Cryo ESEM (FEI Quanta FEG 200)=		=QFEG 200 Cryo ESEM (FEI Quanta FEG 200)=

	[[Image:FEG.jpg\|300x300px\|right\|thumb\| DTU ~~Nanolabs~~ QFEG ~~positioned~~ in building ~~314~~ room ~~no. 010~~]]		[[Image:FEG.jpg\|300x300px\|right\|thumb\| DTU Nanolab QFEG located in building 307 room 907]]

	The QFEG 200 Cryo is an FEI Quanta FEG (Field Emission Gun) scanning electron microscope with a spatial resolution of 2 nm for the ETD detector in high vacuum at 30 keV. The microscope can operate in several modes, such as high vacuum, low vacuum, environmental mode, room temperature, low temperature and cryo. Our QFEG is fitted with an EDS detector, which allows for analytical measurements in all the operation modes. With the cryo transfer system available, it is especially suited for cryo experiments.		The QFEG 200 Cryo is an FEI Quanta FEG (Field Emission Gun) scanning electron microscope with a spatial resolution of 2 nm for the ETD detector in high vacuum at 30 keV. The microscope can operate in several modes, such as high vacuum, low vacuum, environmental mode, room temperature, low temperature and cryo. Our QFEG is fitted with an EDS detector, which allows for analytical measurements in all the operation modes. With the cryo transfer system available, it is especially suited for cryo experiments.

Jenk at 13:53, 27 June 2023

2023-06-27T13:53:02Z

← Older revision		Revision as of 15:53, 27 June 2023
Line 99:		Line 99:

	= Examples of the QFEG's capabilities =		= Examples of the QFEG's capabilities =

			''All images are taken by Marie Karen Tracy Hong Lin @DTU Nanolab''

	== SEM (Scanning Electron Microscopy) ==		== SEM (Scanning Electron Microscopy) ==
	The SEM is a remarkable technique which can offer information such as the surface morphology of a sample and also a 3D appearance of the specimen. It can offer the best resolution (2 nm at 30kV) at high magnification and also topographical details because of its great depth of field.		The SEM is a remarkable technique which can offer information such as the surface morphology of a sample and also a 3D appearance of the specimen. It can offer the best resolution (2 nm at 30kV) at high magnification and also topographical details because of its great depth of field.

Jenk at 08:10, 27 June 2023

2023-06-27T08:10:59Z

← Older revision		Revision as of 10:10, 27 June 2023
Line 1:		Line 1:
	'''Feedback to this page''': '''[mailto:labadviser@nanolab.dtu.dk?Subject=Feed%20back%20from%20page%20http://labadviser.nanolab.dtu.dk/index.php?title=LabAdviser/314/Microscopy_314-307/SEM/QFEG click here]'''		'''Feedback to this page''': '''[mailto:labadviser@nanolab.dtu.dk?Subject=Feed%20back%20from%20page%20http://labadviser.nanolab.dtu.dk/index.php?title=LabAdviser/314/Microscopy_314-307/SEM/QFEG click here]'''

			''This section is written by DTU Nanolab internal if nothing else is stated.''
	[[Category:314]]		[[Category:314]]
	[[Category:314-Microscopy]]		[[Category:314-Microscopy]]

Mktracy: /* EDS (Energy Dispersive X-ray Spectroscopy) */

2023-04-13T14:08:54Z

EDS (Energy Dispersive X-ray Spectroscopy)

← Older revision		Revision as of 16:08, 13 April 2023
Line 133:		Line 133:
	[[File:EDS Bacteria and Au nanoparticle.JPG\|1000px\|left\|thumb\|EDS mapping of bacteria and gold nanoparticle]]<br clear="all" />		[[File:EDS Bacteria and Au nanoparticle.JPG\|1000px\|left\|thumb\|EDS mapping of bacteria and gold nanoparticle]]<br clear="all" />

	[[File:Microalgae with ~~Nanoparticles~~.jpg\|1000px\|left\|thumb\|SEM analysis and EDS mapping of microalgae with iron ~~nanoparticle~~]]<br clear="all" />		[[File:Microalgae with iron nanoparticles .jpg\|1000px\|left\|thumb\|SEM analysis and EDS mapping of microalgae with iron nanoparticles]]<br clear="all" />

Mktracy: /* EDS (Energy Dispersive X-ray Spectroscopy) */

2023-04-13T13:36:01Z

EDS (Energy Dispersive X-ray Spectroscopy)

← Older revision		Revision as of 15:36, 13 April 2023
Line 132:		Line 132:

	[[File:EDS Bacteria and Au nanoparticle.JPG\|1000px\|left\|thumb\|EDS mapping of bacteria and gold nanoparticle]]<br clear="all" />		[[File:EDS Bacteria and Au nanoparticle.JPG\|1000px\|left\|thumb\|EDS mapping of bacteria and gold nanoparticle]]<br clear="all" />

			[[File:Microalgae with Nanoparticles.jpg\|1000px\|left\|thumb\|SEM analysis and EDS mapping of microalgae with iron nanoparticle]]<br clear="all" />

Mktracy: /* SEM (Scanning Electron Microscopy) */

2022-02-17T13:37:58Z

SEM (Scanning Electron Microscopy)

← Older revision		Revision as of 15:37, 17 February 2022
Line 100:		Line 100:
	== SEM (Scanning Electron Microscopy) ==		== SEM (Scanning Electron Microscopy) ==
	The SEM is a remarkable technique which can offer information such as the surface morphology of a sample and also a 3D appearance of the specimen. It can offer the best resolution (2 nm at 30kV) at high magnification and also topographical details because of its great depth of field.		The SEM is a remarkable technique which can offer information such as the surface morphology of a sample and also a 3D appearance of the specimen. It can offer the best resolution (2 nm at 30kV) at high magnification and also topographical details because of its great depth of field.

	[[File:20201017_Microalgae_Image01_018-1.jpg\|500px\|left\|thumb\|SEM imaging of Microalgae with gold nanoparticles]]<br clear="all" />		[[File:20201017_Microalgae_Image01_018-1.jpg\|500px\|left\|thumb\|SEM imaging of Microalgae with gold nanoparticles]]<br clear="all" />
	[[File:20191217 Bacteria Image_032-1.jpg\|500px\|left\|thumb\|SEM imaging of Bacteria]]<br clear="all" />		[[File:20191217 Bacteria Image_032-1.jpg\|500px\|left\|thumb\|SEM imaging of Bacteria]]<br clear="all" />
	[[File:Spider_006-1.jpg\|500px\|left\|thumb\|SEM imaging of Spider]]<br clear="all" />		[[File:Spider_006-1.jpg\|500px\|left\|thumb\|SEM imaging of Spider]]<br clear="all" />
	[[File:Spider Web_020-1.jpg\|500px\|left\|thumb\|SEM imaging of Spider web]]<br clear="all" />		[[File:Spider Web_020-1.jpg\|500px\|left\|thumb\|SEM imaging of Spider web]]<br clear="all" />


	== BSD (Backscattered Electron Detector) ==		== BSD (Backscattered Electron Detector) ==

Mktracy: /* STEM (Scanning Transmission Electron Microscopy) */

2022-02-17T13:26:41Z

STEM (Scanning Transmission Electron Microscopy)

← Older revision		Revision as of 15:26, 17 February 2022
Line 112:		Line 112:

	== STEM (Scanning Transmission Electron Microscopy) ==		== STEM (Scanning Transmission Electron Microscopy) ==
	STEM combines the principles of transmission electron microscopy and scanning electron microscopy and can be performed on either type of instrument. In the STEM setting, the sample requires to have a thickness of less than 200 nm for visualization. Sample preparation prior to STEM microscopy involve samples being embedded in epoxy resin and then ultra ~~section~~ using the [[LabAdviser/314/Preparation_314-307/Soft-matter#Leica_EM_UC7_Ultramicrotome\|Ultramicrotome]]. For more sensitive samples that need to be sectionned at cryogenic temperatures (below −180 °C), the [[LabAdviser/314/Preparation_314-307/Soft-matter#Leica_EM_FC7_Cryo-Ultramicrotome\|Cryo-Ultramicrotome]] can be used for that purpose.		STEM combines the principles of transmission electron microscopy and scanning electron microscopy and can be performed on either type of instrument. In the STEM setting, the sample requires to have a thickness of less than 200 nm for visualization. Sample preparation prior to STEM microscopy involve samples being embedded in epoxy resin and then ultra sectioning using the [[LabAdviser/314/Preparation_314-307/Soft-matter#Leica_EM_UC7_Ultramicrotome\|Ultramicrotome]]. For more sensitive samples that need to be sectionned at cryogenic temperatures (below −180 °C), the [[LabAdviser/314/Preparation_314-307/Soft-matter#Leica_EM_FC7_Cryo-Ultramicrotome\|Cryo-Ultramicrotome]] can be used for that purpose.

	[[File:20210519_Microalgae_K2_007-1.jpg\|500px\|left\|thumb\|STEM imaging of a cross section of Microalgae, 100 nm thick section]]<br clear="all" />		[[File:20210519_Microalgae_K2_007-1.jpg\|500px\|left\|thumb\|STEM imaging of a cross section of Microalgae, 100 nm thick section]]<br clear="all" />

Mktracy: /* STEM (Scanning Transmission Electron Microscopy) */

2022-01-10T12:59:18Z

STEM (Scanning Transmission Electron Microscopy)

← Older revision		Revision as of 14:59, 10 January 2022
Line 112:		Line 112:

	== STEM (Scanning Transmission Electron Microscopy) ==		== STEM (Scanning Transmission Electron Microscopy) ==
	STEM combines the principles of transmission electron microscopy and scanning electron microscopy and can be performed on either type of instrument. In the STEM setting, the sample requires to have a thickness of less than 200 nm for visualization. Sample preparation prior to STEM microscopy involve samples being embedded in epoxy resin and then ultra section using the [[LabAdviser/314/Preparation_314-307/Soft-matter#Leica_EM_UC7_Ultramicrotome\|Ultramicrotome]]. For more sensitive samples that need to be sectionned at cryogenic temperatures (below −180 °C), ~~the thin sections can also be done at Nanolab by using~~ the [[LabAdviser/314/Preparation_314-307/Soft-matter#Leica_EM_FC7_Cryo-Ultramicrotome\|Cryo-Ultramicrotome]].		STEM combines the principles of transmission electron microscopy and scanning electron microscopy and can be performed on either type of instrument. In the STEM setting, the sample requires to have a thickness of less than 200 nm for visualization. Sample preparation prior to STEM microscopy involve samples being embedded in epoxy resin and then ultra section using the [[LabAdviser/314/Preparation_314-307/Soft-matter#Leica_EM_UC7_Ultramicrotome\|Ultramicrotome]]. For more sensitive samples that need to be sectionned at cryogenic temperatures (below −180 °C), the [[LabAdviser/314/Preparation_314-307/Soft-matter#Leica_EM_FC7_Cryo-Ultramicrotome\|Cryo-Ultramicrotome]] can be used for that purpose.

	[[File:20210519_Microalgae_K2_007-1.jpg\|500px\|left\|thumb\|STEM imaging of a cross section of Microalgae, 100 nm thick section]]<br clear="all" />		[[File:20210519_Microalgae_K2_007-1.jpg\|500px\|left\|thumb\|STEM imaging of a cross section of Microalgae, 100 nm thick section]]<br clear="all" />