LabAdviser/314/Preparation 314-307: Difference between revisions
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The FEI Vitrobot™ is used for vitrifying (freezing) single particle samples for cryo-TEM. The Vitrobot™ is fully automated and performs the cryo-fixation process at constant conditions (temperature, relative humidity, blotting and freezing velocity), which ensures high quality cryo-fixation. The sample is pipetted on a TEM grid, blotted and then plunged in liquid ethane, which is cooled with liquid nitrogen. The FEI Vitrobot™ Data Sheet is here [[:File:Vitrobot_Mark_IV_Datasheet.pdf]] and an example of the APV can be found here [[:File:APV_Vitrobot.pdf]]. | The FEI Vitrobot™ is used for vitrifying (freezing) single particle samples for cryo-TEM. The Vitrobot™ is fully automated and performs the cryo-fixation process at constant conditions (temperature, relative humidity, blotting and freezing velocity), which ensures high quality cryo-fixation. The sample is pipetted on a TEM grid, blotted and then plunged in liquid ethane, which is cooled with liquid nitrogen. The FEI Vitrobot™ Data Sheet is here [[:File:Vitrobot_Mark_IV_Datasheet.pdf]] and an example of the APV can be found here [[:File:APV_Vitrobot.pdf]]. | ||
The Vitrobot™ is located in building 314, in the Soft Mater (toxic) Lab. For further information on the machine usage contact ramona.mateiu@cen.dtu.dk. | The Vitrobot™ is located in building 314, in the Soft Mater (toxic) Lab. For further information on the machine usage contact ramona.mateiu@cen.dtu.dk. | ||
[[File:Example.jpg]] | |||
[[File: Leica EM CPD300.jpg|200px|thumb|left|DTU Cens Leica EM CPD300]] | |||
== Microtomes == | == Microtomes == | ||
Revision as of 17:21, 18 January 2016
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Optical Microscopes
1) Olympus BX51 colorview.
2) Olympus SZ61 stereo Zoom Microscope.
3) Leica MZ75 stereo microscope.
File:Operation Manual Leica_MZ75.pdf
Ion milling
1) Fischione Low Angle Ion Milling and Polishing System Model 1010.
File:Operation Manual Fischione_1010.pdf
.
2) Fischione NanoMill model 1040.
Pumping stations
1) Pumping Cube for single TEM specimen holder.
2) Gatan Turbo Pumping Station model 655. Capacity 4 specimen holders.
Polishing
1) Struers Labopol -25.
2) TechPrep Allied.
3) Struer Labopol -5.
4) Struer Labopol -4.
Saws
1) Struers Minitom.
2) Well Precision Diamond Wire Saw.
Plasma cleaner & Pumping Station
1) Fischione Plasma Cleaner model 1020.
.
Coaters & Accessories
1) Cressington 208 Carbon Coater.
2) Cressington 208HR Sputter Coater.
Accessory for 208HR Sputter Coater:
Glow Discharge Head
Sonicators
1) Ultrasonic Branson 2510.
2) VWR Ultrasonic Cleaner.
Hot plates
1) Nuova hot plate.
Plunger
The FEI Vitrobot™ is used for vitrifying (freezing) single particle samples for cryo-TEM. The Vitrobot™ is fully automated and performs the cryo-fixation process at constant conditions (temperature, relative humidity, blotting and freezing velocity), which ensures high quality cryo-fixation. The sample is pipetted on a TEM grid, blotted and then plunged in liquid ethane, which is cooled with liquid nitrogen. The FEI Vitrobot™ Data Sheet is here File:Vitrobot_Mark_IV_Datasheet.pdf and an example of the APV can be found here File:APV_Vitrobot.pdf.
The Vitrobot™ is located in building 314, in the Soft Mater (toxic) Lab. For further information on the machine usage contact ramona.mateiu@cen.dtu.dk.
Microtomes
Critical point dryer
Leica EM CPD300. The Critical Point Drier (CPD) is used for drying samples for the SEM. The machine uses the fact that at the critical point the solvent can be converted from liquid to gas without crossing the interfaces between liquid and gas (no surface tension) and hence no sample drying artefacts. CO2 is the solvent of choice as its triple point is suitable for biological samples (31°C and 74 bars). The CO2 is not miscible with water; therefore the sample should be dissolved in acetone or ethanol. The Leica EM CPD300 operating manual is here CPD030.pdfand the APV here APV- Critical Point Dryer.docx.
The Leica EM CPD300 is located at DTU Cen, building 314, in the Soft Mater (toxic) Lab. For further information on the machine usage contact ramona.mateiu@cen.dtu.dk.